| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SAE1 |
| Gene ID: | 10055 |
| Uniprot ID: | SAE1_HUMAN |
| Immunogen Region: | 1-346 |
| Immunogen Sequence: | MVEKEEAGGGISEEEAAQYD RQIRLWGLEAQKRLRASRVL LVGLKGLGAEIAKNLILAGV KGLTMLDHEQVTPEDPGAQF LIRTGSVGRNRAEASLERAQ NLNPMVDVKVDTEDIEKKPE SFFTQFDAVCLTCCSRDVIV KVDQICHKNSIKFFTGDVFG YHGYTFANLGEHEFVEEKTK VAKVSQGVEDGPDTKRAKLD SSETTMVKKKVVFCPVKEAL EVDWSSEKAKAALKRTTSD |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human SAE1 (NP_005491.1). |
| Tissue Specificity | Expression level increases during S phase and drops in G2 phase (at protein level). |
| Function | The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. |
| Protein Name | Sumo-Activating Enzyme Subunit 1Ubiquitin-Like 1-Activating Enzyme E1a Cleaved Into - Sumo-Activating Enzyme Subunit 1 - N-Terminally Processed |
| Database Links | Reactome: R-HSA-3065676Reactome: R-HSA-3065678 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Sumo-Activating Enzyme Subunit 1 antibodyAnti-Ubiquitin-Like 1-Activating Enzyme E1a Cleaved Into - Sumo-Activating Enzyme Subunit 1 - N-Terminally Processed antibodyAnti-SAE1 antibodyAnti-AOS1 antibodyAnti-SUA1 antibodyAnti-UBLE1A antibody |
Information sourced from Uniprot.org
![Immunofluorescence analysis of HeLa cells using [KO Validated] ANXA1 Rabbit polyclonal antibody (STJ22617) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/99133/383742/STJ22617_1__10539.1713152308.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KO Validated] ANXA1 Rabbit polyclonal antibody (STJ22617) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
