• Western blot analysis of extracts of various cell lines, using SAE1 antibody (STJ112001) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using SAE1 antibody (STJ112001) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SAE1 antibody (1-346) (STJ112001)

SKU:
STJ112001

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SAE1 (1-346) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SAE1
Gene ID: 10055
Uniprot ID: SAE1_HUMAN
Immunogen Region: 1-346
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human SAE1 (NP_005491.1).
Immunogen Sequence: MVEKEEAGGGISEEEAAQYD RQIRLWGLEAQKRLRASRVL LVGLKGLGAEIAKNLILAGV KGLTMLDHEQVTPEDPGAQF LIRTGSVGRNRAEASLERAQ NLNPMVDVKVDTEDIEKKPE SFFTQFDAVCLTCCSRDVIV KVDQICHKNSIKFFTGDVFG YHGYTFANLGEHEFVEEKTK VAKVSQGVEDGPDTKRAKLD SSETTMVKKKVVFCPVKEAL EVDWSSEKAKAALKRTTSD
Tissue Specificity Expression level increases during S phase and drops in G2 phase (at protein level).
Function The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Protein Name Sumo-Activating Enzyme Subunit 1
Ubiquitin-Like 1-Activating Enzyme E1a Cleaved Into - Sumo-Activating Enzyme Subunit 1 - N-Terminally Processed
Database Links Reactome: R-HSA-3065676
Reactome: R-HSA-3065678
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Sumo-Activating Enzyme Subunit 1 antibody
Anti-Ubiquitin-Like 1-Activating Enzyme E1a Cleaved Into - Sumo-Activating Enzyme Subunit 1 - N-Terminally Processed antibody
Anti-SAE1 antibody
Anti-AOS1 antibody
Anti-SUA1 antibody
Anti-UBLE1A antibody

Information sourced from Uniprot.org

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