• Western blot analysis of extracts of various cell lines, using S100P antibody (STJ116980) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using S100P antibody (STJ116980) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human placenta using S100P antibody (STJ116980) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse testis using S100P antibody (STJ116980) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of human placenta cells using S100P rabbit polyclonal antibody (STJ116980) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300ug extracts of HT-29 cells using 3ug S100P antibody (STJ116980). Western blot was performed from the immunoprecipitate using S100P antibody (STJ116980) at a dilition of 1:1000.

Anti-S100P antibody (1-95) (STJ116980)

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STJ116980

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-S100P (1-95) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:100-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: S100P
Gene ID: 6286
Uniprot ID: S100P_HUMAN
Immunogen Region: 1-95
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-95 of human S100P (NP_005971.1).
Immunogen Sequence: MTELETAMGMIIDVFSRYSG SEGSTQTLTKGELKVLMEKE LPGFLQSGKDKDAVDKLLKD LDANGDAQVDFSEFIVFVAA ITSACHKYFEKAGLK
Tissue Specificity Detected in all of the tissues except brain, testis and small intestine, expression level is higher in placenta, heart, lung, skeletal muscle, spleen and leukocyte. Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias.
Function May function as calcium sensor and contribute to cellular calcium signaling. In a calcium-dependent manner, functions by interacting with other proteins, such as EZR and PPP5C, and indirectly plays a role in physiological processes like the formation of microvilli in epithelial cells. May stimulate cell proliferation in an autocrine manner via activation of the receptor for activated glycation end products (RAGE).
Protein Name Protein S100-P
Migration-Inducing Gene 9 Protein
Mig9
Protein S100-E
S100 Calcium-Binding Protein P
Database Links Reactome: R-HSA-6798695
Cellular Localisation Nucleus
Cytoplasm
Cell Projection
Microvillus Membrane
Colocalizes With S100pbp In The Nucleus
Colocolizes With Ezr In The Microvilli In A Calcium-Dependent Manner
Alternative Antibody Names Anti-Protein S100-P antibody
Anti-Migration-Inducing Gene 9 Protein antibody
Anti-Mig9 antibody
Anti-Protein S100-E antibody
Anti-S100 Calcium-Binding Protein P antibody
Anti-S100P antibody
Anti-S100E antibody

Information sourced from Uniprot.org

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