Anti-RYBP antibody [R09-8H5] (STJA0036837)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human RYBP
General Information
| Short Description | Rabbit monoclonal anti-RYBP for use in WB, IHC-P, ICC, IF, IP and FC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF/IP/FC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R09-8H5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:50FC 1:50-1:100 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | RYBP |
| Gene ID | 23429 |
| Uniprot ID | RYBP_HUMAN |
| Immunogen | A synthesized peptide derived from human RYBP |
Additional Info
| Tissue Specificity | Down-regulated in breast cancer tissues and in several breast cancer cell lines (at protein level). Widely expressed with highest levels in lymphoid tissues and placenta. |
| Post Translational Modifications | Monoubiquitinated. |
| Function | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones.it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females. May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites. Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. May play a role in the repression of tumor growth and metastasis in breast cancer by down-regulating SRRM3. |
| Protein Name | Ring1 And Yy1-Binding ProteinApoptin-Associating Protein 1Apap-1Death Effector Domain-Associated FactorDed-Associated FactorYy1 And E4tf1-Associated Factor 1 |
| Database Links | Reactome: R-HSA-8939243Reactome: R-HSA-8953750 |
| Cellular Localisation | NucleusCytoplasmNucleoplasmPrimarily Found In The NucleusDetected In A Punctate Pattern Likely To Represent Polycomb Group (Pcg) Bodies |
| Alternative Antibody Names | Anti-Ring1 And Yy1-Binding Protein antibodyAnti-Apoptin-Associating Protein 1 antibodyAnti-Apap-1 antibodyAnti-Death Effector Domain-Associated Factor antibodyAnti-Ded-Associated Factor antibodyAnti-Yy1 And E4tf1-Associated Factor 1 antibodyAnti-RYBP antibodyAnti-DEDAF antibodyAnti-YEAF1 antibody |
Information sourced from Uniprot.org