• Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 3 Mu g RPS24 antibody ( STJ114017). Western blot was performed from the immunoprecipitate using RPS24 antibody ( STJ114017) at a dilution of 1:1000.
  • Immunohistochemistry analysis of RPS24 in paraffin-embedded rat brain using RPS24 Rabbit polyclonal antibody (STJ114017) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using RPS24 Rabbit polyclonal antibody (STJ114017) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RPS24 antibody (1-130) (STJ114017)

SKU:
STJ114017

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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPS24 (1-130) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPS24
Gene ID: 6229
Uniprot ID: RS24_HUMAN
Immunogen Region: 1-130
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human RPS24 (NP_148982.1).
Immunogen Sequence: MNDTVTIRTRKFMTNRLLQR KQMVIDVLHPGKATVPKTEI REKLAKMYKTTPDVIFVFGF RTHFGGGKTTGFGMIYDSLD YAKKNEPKHRLARHGLYEKK KTSRKQRKERKNRMKKVRGT AKANVGAGKK
Tissue Specificity Mature tissues, such as adult brain, skeletal muscle, heart, and kidney, express low levels, whereas tissues and organs with significant populations of proliferating cells, such as fetal brain, placenta, bone marrow, and various glandular organs, contain significantly higher levels.
Function Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Required for processing of pre-rRNA and maturation of 40S ribosomal subunits. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
Protein Name Small Ribosomal Subunit Protein Es24
40s Ribosomal Protein S24
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72649
Reactome: R-HSA-72689
Reactome: R-HSA-72695
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-9735869
Reactome: R-HSA-9754678
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Nucleus
Nucleolus
Alternative Antibody Names Anti-Small Ribosomal Subunit Protein Es24 antibody
Anti-40s Ribosomal Protein S24 antibody
Anti-RPS24 antibody

Information sourced from Uniprot.org

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