• Confocal immunofluorescence analysis of HeLa cells using RPL36 Polyclonal Antibody (STJ110103) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Confocal immunofluorescence analysis of HeLa cells using RPL36 antibody (STJ110103) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using RPL36 antibody (STJ110103) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using RPL36 Rabbit polyclonal antibody (STJ110103) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using RPL36 Rabbit polyclonal antibody (STJ110103) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using RPL36 Rabbit polyclonal antibody (STJ110103) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using RPL36 antibody (STJ110103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RPL36 antibody (1-105) (STJ110103)

SKU:
STJ110103

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPL36 (1-105) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPL36
Gene ID: 25873
Uniprot ID: RL36_HUMAN
Immunogen Region: 1-105
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human RPL36 (NP_056229.2).
Immunogen Sequence: MALRYPMAVGLNKGHKVTKN VSKPRHSRRRGRLTKHTKFV RDMIREVCGFAPYERRAMEL LKVSKDKRALKFIKKRVGTH IRAKRKREELSNVLAAMRKA AAKKD
Function Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Protein Name Large Ribosomal Subunit Protein El36
60s Ribosomal Protein L36
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72689
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Cytosol
Detected On Cytosolic Polysomes
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein El36 antibody
Anti-60s Ribosomal Protein L36 antibody
Anti-RPL36 antibody

Information sourced from Uniprot.org

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