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Anti-RPL21 antibody (1-160) (STJ116467)

Confocal immunofluorescence analysis of A-431 cells using RPL21 Rabbit polyclonal antibody (STJ116467) at dilution of 1:100. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of HeLa cells using RPL21 Rabbit polyclonal antibody (STJ116467) at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of RPL21 in paraffin-embedded rat brain using RPL21 Rabbit polyclonal antibody (STJ116467) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Western blot analysis of various lysates using RPL21 Rabbit polyclonal antibody (STJ116467) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-RPL21 antibody (1-160) (STJ116467)

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STJ116467

£169.50 - £449.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-RPL21 (1-160) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000 IHC-P 1:50-1:100 IF/ICC 1:50-1:100
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	RPL21
Gene ID:	6144
Uniprot ID:	RL21_HUMAN
Immunogen Region:	1-160
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human RPL21 (NP_000973.2).
Immunogen Sequence:	MTNTKGKRRGTRYMFSRPFR KHGVVPLATYMRIYKKGDIV DIKGMGTVQKGMPHKCYHGK TGRVYNVTQHAVGIVVNKQV KGKILAKRINVRIEHIKHSK SRDSFLKRVKENDQKKKEAK EKGTWVQLKRQPAPPREAHF VRTNGKEPELLEPIPYEFMA

Function	Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Protein Name	Large Ribosomal Subunit Protein El21 60s Ribosomal Protein L21
Database Links	Reactome: R-HSA-156827 Reactome: R-HSA-156902 Reactome: R-HSA-1799339 Reactome: R-HSA-192823 Reactome: R-HSA-2408557 Reactome: R-HSA-6791226 Reactome: R-HSA-72689 Reactome: R-HSA-72706 Reactome: R-HSA-72764 Reactome: R-HSA-9010553 Reactome: R-HSA-9633012 Reactome: R-HSA-975956 Reactome: R-HSA-975957
Cellular Localisation	Cytoplasm Cytosol Endoplasmic Reticulum Detected On Cytosolic Polysomes Detected In Ribosomes That Are Associated With The Rough Endoplasmic Reticulum
Alternative Antibody Names	Anti-Large Ribosomal Subunit Protein El21 antibody Anti-60s Ribosomal Protein L21 antibody Anti-RPL21 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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