• Western blot analysis of extracts of Rat testis, using RPGR antibody (STJ25388) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of mouse brain, using RPGR antibody (STJ25388) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using RPGR rabbit polyclonal antibody (STJ25388) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using RPGR rabbit polyclonal antibody (STJ25388) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using RPGR rabbit polyclonal antibody (STJ25388) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of mouse retina cells using RPGR rabbit polyclonal antibody (STJ25388) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat retina cells using RPGR rabbit polyclonal antibody (STJ25388) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-RPGR antibody (1-260) (STJ25388)

SKU:
STJ25388

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPGR (1-260) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPGR
Gene ID: 6103
Uniprot ID: RPGR_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human RPGR (NP_001030025.1).
Immunogen Sequence: MREPEELMPDSGAVFTFGKS KFAENNPGKFWFKNDVPVHL SCGDEHSAVVTGNNKLYMFG SNNWGQLGLGSKSAISKPTC VKALKPEKVKLAACGRNHTL VSTEGGNVYATGGNNEGQLG LGDTEERNTFHVISFFTSEH KIKQLSAGSNTSAALTEDGR LFMWGDNSEGQIGLKNVSNV CVPQQVTIGKPVSWISCGYY HSAFVTTDGELYVFGEPENG KLGLPNQLLGNHRTPQLVS
Tissue Specificity Heart, brain, placenta, lung, liver, muscle, kidney, retina, pancreas and fetal retinal pigment epithelium. Isoform 3 is found only in the retina. Colocalizes with RPGRIP1 in the outer segment of rod photoreceptors and cone outer segments.
Post Translational Modifications Prenylated.
Function Could be a guanine-nucleotide releasing factor. Plays a role in ciliogenesis. Probably regulates cilia formation by regulating actin stress filaments and cell contractility. Plays an important role in photoreceptor integrity. May play a critical role in spermatogenesis and in intraflagellar transport processes. May be involved in microtubule organization and regulation of transport in primary cilia.
Protein Name X-Linked Retinitis Pigmentosa Gtpase Regulator
Cellular Localisation Cytoplasm
Cytoskeleton
Flagellum Axoneme
Golgi Apparatus
Cell Projection
Cilium
In The Retinal Photoreceptor Cell Layer
Localizes At The Connecting Cilium
Colocalizes With Whrn In The Photoreceptor Connecting Cilium
Colocalizes With Cep290 In The Photoreceptor Connecting Cilium
Colocalizes With Rpgrip1 In The Photoreceptor Connecting Cilium
Isoform 6: Cytoplasm
Microtubule Organizing Center
Centrosome
Cilium Basal Body
Cilium Axoneme
Alternative Antibody Names Anti-X-Linked Retinitis Pigmentosa Gtpase Regulator antibody
Anti-RPGR antibody
Anti-RP3 antibody
Anti-XLRP3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance