Anti-ROBO1/DUTT1 antibody (Internal) (STJ70835)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ROBO1/DUTT1 (Internal) is suitable for use in ELISA, Immunofluorescence and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/IF/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:16000.IHC: Paraffin embedded Human Brain (Cortex) and Kidney. Recommended concentration: 5-6µg/ml.IF: Strong expression of the protein seen in the Plasma membrane and Golgi/ER of HeLa and U |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ROBO1 |
| Gene ID | 6091 |
| Uniprot ID | ROBO1_HUMAN |
| Accession Number | NP_002932.1; NP_598334.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | GDVDLSNKINEMK |
| Specificity | Not expected to cross-react with ROBO2 or ROBO3. |
Additional Info
| Post Translational Modifications | Ubiquitinated. May be deubiquitinated by USP33. |
| Function | Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B.inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development. |
| Protein Name | Roundabout Homolog 1Deleted In U Twenty TwentyH-Robo-1 |
| Database Links | Reactome: R-HSA-373752Reactome: R-HSA-376176Reactome: R-HSA-428540Reactome: R-HSA-428542Reactome: R-HSA-428543Reactome: R-HSA-428890Reactome: R-HSA-8985586Reactome: R-HSA-8985801Reactome: R-HSA-9010553 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionAxonEndoplasmic Reticulum-Golgi Intermediate Compartment MembraneSingle-Pass Membrane ProteinDetected At Growth Cones In Thalamus NeuronsPrrg4 Prevents Cell Surface Location And Both Colocalize In The Endoplasmic Reticulum/Golgi Adjacent To The Cell Nucleus |
| Alternative Antibody Names | Anti-Roundabout Homolog 1 antibodyAnti-Deleted In U Twenty Twenty antibodyAnti-H-Robo-1 antibodyAnti-ROBO1 antibodyAnti-DUTT1 antibody |
Information sourced from Uniprot.org