• STJ70835 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Golgi and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70835 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • STJ70835 (1µg/ml) staining of HUVEC lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ROBO1/DUTT1 antibody (Internal) (STJ70835)

SKU:
STJ70835-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/IHC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ROBO1/DUTT1 (Internal) is suitable for use in ELISA, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-2µg/ml
IF-Strong expression of the protein seen in the Golgi and membranes of HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ROBO1
Gene ID: 6091
Uniprot ID: ROBO1_HUMAN
Immunogen Region: Internal
Accession Number: NP_002932.1; NP_598334.1
Specificity: Not expected to cross-react with ROBO2 or ROBO3.
Immunogen Sequence: GDVDLSNKINEMK
Post Translational Modifications Ubiquitinated. May be deubiquitinated by USP33.
Function Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B.inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development.
Protein Name Roundabout Homolog 1
Deleted In U Twenty Twenty
H-Robo-1
Database Links Reactome: R-HSA-373752
Reactome: R-HSA-376176
Reactome: R-HSA-428540
Reactome: R-HSA-428542
Reactome: R-HSA-428543
Reactome: R-HSA-428890
Reactome: R-HSA-8985586
Reactome: R-HSA-8985801
Reactome: R-HSA-9010553
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Axon
Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Single-Pass Membrane Protein
Detected At Growth Cones In Thalamus Neurons
Prrg4 Prevents Cell Surface Location And Both Colocalize In The Endoplasmic Reticulum/Golgi Adjacent To The Cell Nucleus
Alternative Antibody Names Anti-Roundabout Homolog 1 antibody
Anti-Deleted In U Twenty Twenty antibody
Anti-H-Robo-1 antibody
Anti-ROBO1 antibody
Anti-DUTT1 antibody

Information sourced from Uniprot.org

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