• Western blot analysis of extracts of various cell lines, using RNASE2 antibody (STJ111990) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of paraffin-embedded mouse spleen using RNASE2 Rabbit polyclonal antibody (STJ111990) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse spleen using RNASE2 rabbit polyclonal antibody (STJ111990) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using RNASE2 Rabbit polyclonal antibody (STJ111990) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RNASE2 antibody (28-161) (STJ111990)

SKU:
STJ111990

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RNASE2 (28-161) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RNASE2
Gene ID: 6036
Uniprot ID: RNAS2_HUMAN
Immunogen Region: 28-161
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 28-161 of human RNASE2 (NP_002925.1).
Immunogen Sequence: KPPQFTWAQWFETQHINMTS QQCTNAMQVINNYQRRCKNQ NTFLLTTFANVVNVCGNPNM TCPSNKTRKNCHHSGSQVPL IHCNLTTPSPQNISNCRYAQ TPANMFYIVACDNRDQRRDP PQYPVVPVHLDRII
Tissue Specificity Liver, lung, spleen, leukocytes and body fluids.
Post Translational Modifications A particular signal processing and glycosylation pattern may differentiate the UpI2 RNase, found specifically in pregnant women urine, from other nonsecretory RNases.
Function This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities.
Protein Name Non-Secretory Ribonuclease
Eosinophil-Derived Neurotoxin
Rnase Upi-2
Ribonuclease 2
Rnase 2
Ribonuclease Us
Database Links Reactome: R-HSA-6798695
Cellular Localisation Lysosome
Cytoplasmic Granule
Matrix Of Eosinophil's Large Specific Granule
Alternative Antibody Names Anti-Non-Secretory Ribonuclease antibody
Anti-Eosinophil-Derived Neurotoxin antibody
Anti-Rnase Upi-2 antibody
Anti-Ribonuclease 2 antibody
Anti-Rnase 2 antibody
Anti-Ribonuclease Us antibody
Anti-RNASE2 antibody
Anti-EDN antibody
Anti-RNS2 antibody

Information sourced from Uniprot.org

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