• Western blot analysis of mouse liver, mouse kidney cells using RFC1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-RFC1 antibody (Internal) (STJ96679)

SKU:
STJ96679

Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Replication factor C subunit 1 (Internal) is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RFC1
Gene ID: 5981
Uniprot ID: RFC1_HUMAN
Immunogen Region: Internal
Specificity: RFC1 Polyclonal Antibody detects endogenous levels of RFC1 protein.
Immunogen: Synthesized peptide derived from the Internal region of human RFC1.
Function The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA. Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
Protein Name Replication Factor C Subunit 1
Activator 1 140 Kda Subunit
A1 140 Kda Subunit
Activator 1 Large Subunit
Activator 1 Subunit 1
Dna-Binding Protein Po-Ga
Replication Factor C 140 Kda Subunit
Rf-C 140 Kda Subunit
Rfc140
Replication Factor C Large Subunit
Database Links Reactome: R-HSA-110312
Reactome: R-HSA-110314
Reactome: R-HSA-110320
Reactome: R-HSA-174411
Reactome: R-HSA-5651801
Reactome: R-HSA-5655862
Reactome: R-HSA-5656121
Reactome: R-HSA-5656169
Reactome: R-HSA-5685942
Reactome: R-HSA-5696397
Reactome: R-HSA-5696400
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-69091
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Replication Factor C Subunit 1 antibody
Anti-Activator 1 140 Kda Subunit antibody
Anti-A1 140 Kda Subunit antibody
Anti-Activator 1 Large Subunit antibody
Anti-Activator 1 Subunit 1 antibody
Anti-Dna-Binding Protein Po-Ga antibody
Anti-Replication Factor C 140 Kda Subunit antibody
Anti-Rf-C 140 Kda Subunit antibody
Anti-Rfc140 antibody
Anti-Replication Factor C Large Subunit antibody
Anti-RFC1 antibody
Anti-RFC140 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance