• Immunohistochemistry analysis of paraffin-embedded rat heart using REG3A antibody (STJ114353) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using REG3A antibody (STJ114353) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using REG3A antibody (STJ114353) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using REG3A antibody (STJ114353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-REG3A antibody (27-175) (STJ114353)

SKU:
STJ114353

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-REG3A (27-175) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: REG3A
Gene ID: 5068
Uniprot ID: REG3A_HUMAN
Immunogen Region: 27-175
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-175 of human REG3A (NP_002571.1).
Immunogen Sequence: EEPQRELPSARIRCPKGSKA YGSHCYALFLSPKSWTDADL ACQKRPSGNLVSVLSGAEGS FVSSLVKSIGNSYSYVWIGL HDPTQGTEPNGEGWEWSSSD VMNYFAWERNPSTISSPGHC ASLSRSTAFLRWKDYNCNVR LPYVCKFTD
Tissue Specificity Expressed by keratinocytes. Highly expressed in epidermal keratinocytes of psoriasis patients (at protein level). Constitutively expressed in intestine. Low expression is found in healthy pancreas. Overexpressed during the acute phase of pancreatitis and in some patients with chronic pancreatitis.
Post Translational Modifications Proteolytic processing by trypsin removes an inhibitory N-terminal propeptide and is essential for peptidoglycan binding and antibacterial activity.
Function Regenerating islet-derived protein 3-alpha 15 kDa form: Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Binds membrane phospholipids and kills bacteria by forming a hexameric membrane-permeabilizing oligomeric pore. Acts as a hormone in response to different stimuli like anti-inflammatory signals, such as IL17A, or gut microbiome. Secreted by different cell types to activate its receptor EXTL3 and induce cell specific signaling pathways. Induced by IL17A in keratinocytes, regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway. In parallel, inhibits skin inflammation through the inhibition of inflammatory cytokines such as IL6 and TNF. In pancreas, is able to permealize beta-cells membrane and stimulate their proliferation. Regenerating islet-derived protein 3-alpha 16.5 kDa form: Has bacteriostatic activity.
Protein Name Regenerating Islet-Derived Protein 3-Alpha
Reg-3-Alpha
Hepatointestinal Pancreatic Protein
Hip/Pap
Human Proislet Peptide
Hip
Pancreatitis-Associated Protein 1
Regenerating Islet-Derived Protein Iii-Alpha
Reg Iii-Alpha Cleaved Into - Regenerating Islet-Derived Protein 3-Alpha 16.5 Kda Form - Regenerating Islet-Derived Protein 3-Alpha 15 Kda Form
Database Links Reactome: R-HSA-6803157
Cellular Localisation Secreted
Found In The Apical Region Of Pancreatic Acinar Cells
Alternative Antibody Names Anti-Regenerating Islet-Derived Protein 3-Alpha antibody
Anti-Reg-3-Alpha antibody
Anti-Hepatointestinal Pancreatic Protein antibody
Anti-Hip/Pap antibody
Anti-Human Proislet Peptide antibody
Anti-Hip antibody
Anti-Pancreatitis-Associated Protein 1 antibody
Anti-Regenerating Islet-Derived Protein Iii-Alpha antibody
Anti-Reg Iii-Alpha Cleaved Into - Regenerating Islet-Derived Protein 3-Alpha 16.5 Kda Form - Regenerating Islet-Derived Protein 3-Alpha 15 Kda Form antibody
Anti-REG3A antibody
Anti-HIP antibody
Anti-PAP antibody
Anti-PAP1 antibody

Information sourced from Uniprot.org

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