• Western blot analysis of extracts of various cell lines, using RDM1 antibody (STJ28927) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RDM1 antibody (125-284) (STJ28927)

SKU:
STJ28927

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RDM1 (125-284) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RDM1
Gene ID: 201299
Uniprot ID: RDM1_HUMAN
Immunogen Region: 125-284
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 125-284 of human RDM1 (NP_663629.1).
Immunogen Sequence: NGCSKRIIKLQELSDLEERE NEDSMVPLPKQSLKFFCALE VVLPSCDCRSPGIGLVEEPM DKVEEGPLSFLMKRKTAQKL AIQKALSDAFQKLLIVVLES GKIAVEYRPSEDIVGVRCEE ELHGLIQVPCSPWKQYGQEE EGYLSDFSLEEEEFRLPELD
Tissue Specificity Expressed in testis.
Function May confer resistance to the antitumor agent cisplatin. Binds to DNA and RNA.
Protein Name Rad52 Motif-Containing Protein 1
Rad52 Homolog B
Cellular Localisation Nucleus
Cytoplasm
Nucleolus
Isoform 3 And Isoform 10 Are Predominantly Nuclear And Nucleolar
After Treatment With Proteasomal Inhibitors And Mild Heat-Shock Stress
Isoform 1
Isoform 3
Isoform 5
Isoform 7
Isoform 8 And Isoform 10 Are Relocalized To The Nucleolus As Dot-Like Or Irregular Subnuclear Structures
Isoform 1 Colocalized With Nuclear Promyelocytic Leukemia (Pml) And Cajal Bodies (Cb)
This Association With Nuclear Bodies Is Enhanced In Response To Proteotoxic Stress
But Not Isoform 1 And Isoform 5
Is Relocalized In Nucleolar Caps During Transcriptional Arrest
Isoform 1: Cytoplasm
Pml Body
Cajal Body
Isoform 1 Is Predominantly Cytoplasmic
Alternative Antibody Names Anti-Rad52 Motif-Containing Protein 1 antibody
Anti-Rad52 Homolog B antibody
Anti-RDM1 antibody
Anti-RAD52B antibody

Information sourced from Uniprot.org

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