| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RCC1 (1-240) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RCC1 |
| Gene ID: |
1104 |
| Uniprot ID: |
RCC1_HUMAN |
| Immunogen Region: |
1-240 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RCC1 (NP_001260.1). |
| Immunogen Sequence: |
MSPKRIAKRRSPPADAIPKS KKVKVSHRSHSTEPGLVLTL GQGDVGQLGLGENVMERKKP ALVSIPEDVVQAEAGGMHTV CLSKSGQVYSFGCNDEGALG RDTSVEGSEMVPGKVELQEK VVQVSAGDSHTAALTDDGRV FLWGSFRDNNGVIGLLEPMK KSMVPVQVQLDVPVVKVASG NDHLVMLTADGDLYTLGCGE QGQLGRVPELFANRGGRQGL ERLLVPKCVMLKSRGSRGH |
| Post Translational Modifications | N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di- and trimethylation produce a permanent positive charge on the amino group, which facilitates electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran. |
| Function | Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis. Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis. Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. |
| Protein Name | Regulator Of Chromosome CondensationCell Cycle Regulatory ProteinChromosome Condensation Protein 1 |
| Database Links | Reactome: R-HSA-165054Reactome: R-HSA-180746Reactome: R-HSA-9615933 |
| Cellular Localisation | NucleusChromosomeCytoplasmPredominantly Nuclear In Interphase CellsBinds To Mitotic Chromosomes |
| Alternative Antibody Names | Anti-Regulator Of Chromosome Condensation antibodyAnti-Cell Cycle Regulatory Protein antibodyAnti-Chromosome Condensation Protein 1 antibodyAnti-RCC1 antibodyAnti-CHC1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
