Anti-RCC1 antibody (1-240) (STJ28433)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RCC1 (1-240) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RCC1 |
| Gene ID | 1104 |
| Uniprot ID | RCC1_HUMAN |
| Immunogen Region | 1-240 |
| Immunogen Sequence | MSPKRIAKRRSPPADAIPKS KKVKVSHRSHSTEPGLVLTL GQGDVGQLGLGENVMERKKP ALVSIPEDVVQAEAGGMHTV CLSKSGQVYSFGCNDEGALG RDTSVEGSEMVPGKVELQEK VVQVSAGDSHTAALTDDGRV FLWGSFRDNNGVIGLLEPMK KSMVPVQVQLDVPVVKVASG NDHLVMLTADGDLYTLGCGE QGQLGRVPELFANRGGRQGL ERLLVPKCVMLKSRGSRGH |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human RCC1 (NP_001260.1). |
Additional Info
| Post Translational Modifications | N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di- and trimethylation produce a permanent positive charge on the amino group, which facilitates electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran. |
| Function | Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis. Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis. Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. |
| Protein Name | Regulator Of Chromosome CondensationCell Cycle Regulatory ProteinChromosome Condensation Protein 1 |
| Database Links | Reactome: R-HSA-165054Reactome: R-HSA-180746Reactome: R-HSA-9615933 |
| Cellular Localisation | NucleusChromosomeCytoplasmPredominantly Nuclear In Interphase CellsBinds To Mitotic Chromosomes |
| Alternative Antibody Names | Anti-Regulator Of Chromosome Condensation antibodyAnti-Cell Cycle Regulatory Protein antibodyAnti-Chromosome Condensation Protein 1 antibodyAnti-RCC1 antibodyAnti-CHC1 antibody |
Information sourced from Uniprot.org