• Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using RBBP9 Rabbit polyclonal antibody (STJ26678) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using RBBP9 Rabbit polyclonal antibody (STJ26678) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using RBBP9 Rabbit polyclonal antibody (STJ26678) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using RBBP9 Rabbit polyclonal antibody (STJ26678) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using RBBP9 antibody (STJ26678) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RBBP9 antibody (1-186) (STJ26678)

SKU:
STJ26678

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RBBP9 (1-186) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RBBP9
Gene ID: 10741
Uniprot ID: RBBP9_HUMAN
Immunogen Region: 1-186
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human RBBP9 (NP_006597.2).
Immunogen Sequence: MASPSKAVIVPGNGGGDVTT HGWYGWVKKELEKIPGFQCL AKNMPDPITARESIWLPFME TELHCDEKTIIIGHSSGAIA AMRYAETHRVYAIVLVSAYT SDLGDENERASGYFTRPWQW EKIKANCPYIVQFGSTDDPF LPWKEQQEVADRLETKLHKF TDCGHFQNTEFHELITVVKS LLKVPA
Tissue Specificity Expressed at higher levels in tumor tissues such as carcinoma.
Function Serine hydrolase whose substrates have not been identified yet. May negatively regulate basal or autocrine TGF-beta signaling by suppressing SMAD2-SMAD3 phosphorylation. May play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-beta through interaction with RB1 and the subsequent displacement of E2F1.
Protein Name Serine Hydrolase Rbbp9
B5t-Overexpressed Gene Protein
Protein Bog
Retinoblastoma-Binding Protein 10
Rbbp-10
Retinoblastoma-Binding Protein 9
Rbbp-9
Alternative Antibody Names Anti-Serine Hydrolase Rbbp9 antibody
Anti-B5t-Overexpressed Gene Protein antibody
Anti-Protein Bog antibody
Anti-Retinoblastoma-Binding Protein 10 antibody
Anti-Rbbp-10 antibody
Anti-Retinoblastoma-Binding Protein 9 antibody
Anti-Rbbp-9 antibody
Anti-RBBP9 antibody
Anti-BOG antibody
Anti-RBBP10 antibody

Information sourced from Uniprot.org

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