• Western blot analysis of extracts of various lysates, using Rad51D antibody (STJ11104088) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RAD51D antibody (1-120) [S4088RM] (STJ11104088)

SKU:
STJ11104088

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Dna Repair Protein Rad51 Homolog 4 (1-120) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4088RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAD51D
Gene ID: 5892
Uniprot ID: RA51D_HUMAN
Immunogen Region: 1-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Rad51D (O75771).
Immunogen Sequence: MGVLRVGLCPGLTEEMIQLL RSHRIKTVVDLVSADLEEVA QKCGLSYKALVALRRVLLAQ FSAFPVNGADLYEELKTSTA ILSTGIGSLDKLLDAGLYTG EVTEIVGGPGSGKTQVCLCM
Tissue Specificity Expressed in colon, prostate, spleen, testis, ovary, thymus and small intestine. Weakly expressed in leukocytes.
Function Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM.
Protein Name Dna Repair Protein Rad51 Homolog 4
R51h3
Rad51 Homolog D
Rad51-Like Protein 3
Trad
Database Links Reactome: R-HSA-5685942
Reactome: R-HSA-5693554
Reactome: R-HSA-5693568
Reactome: R-HSA-5693579
Reactome: R-HSA-5693616
Reactome: R-HSA-6796648
Reactome: R-HSA-9701192
Reactome: R-HSA-9704331
Reactome: R-HSA-9704646
Reactome: R-HSA-9709603
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Chromosome
Telomere
Alternative Antibody Names Anti-Dna Repair Protein Rad51 Homolog 4 antibody
Anti-R51h3 antibody
Anti-Rad51 Homolog D antibody
Anti-Rad51-Like Protein 3 antibody
Anti-Trad antibody
Anti-RAD51D antibody
Anti-RAD51L3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance