• Western blot analysis of extracts of various cell lines, using Rad21 antibody (STJ11101535) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of C6 cells, using Rad21 antibody (STJ11101535) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Rad21 rabbit polyclonal antibody (STJ11101535) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse stomach using Rad21 rabbit polyclonal antibody (STJ11101535) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat stomach using Rad21 rabbit polyclonal antibody (STJ11101535) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U2OS cells using Rad21 rabbit polyclonal antibody (STJ11101535) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g Rad21 antibody (STJ11101535). Western blot was performed from the immunoprecipitate using Rad21 antibody (STJ11101535) at a dilution of 1:1000.
  • Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g Rad21 antibody (STJ11101535). Western blot was performed from the immunoprecipitate using Rad21 antibody (STJ11101535) at a dilution of 1:1000.
  • Chromatin immunoprecipitation analysis of extracts of A-549 cells, using Rad21 antibody (STJ11101535) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Anti-RAD21 antibody (281-631) (STJ11101535)

Anti-RAD21 antibody (281-631) (STJ11101535)

SKU:
STJ11101535

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAD21 (281-631) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
ChIP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAD21
Gene ID: 5885
Uniprot ID: RAD21_HUMAN
Immunogen Region: 281-631
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 281-631 of human Rad21 (NP_006256.1).
Immunogen Sequence: VDPVEPMPTMTDQTTLVPNE EEAFALEPIDITVKETKAKR KRKLIVDSVKELDSKTIRAQ LSDYSDIVTTLDLAPPTKKL MMWKETGGVEKLFSLPAQPL WNNRLLKLFTRCLTPLVPED LRKRRKGGEADNLDEFLKEF
Tissue Specificity Expressed in the gut (at protein level).
Post Translational Modifications Cleaved by separase/ESPL1 at the onset of anaphase.this cleavage is required for sister chromatid separation and cytokinesis. Cleaved by caspase-3/CASP3 or caspase-7/CASP7 at the beginning of apoptosis. Phosphorylated.becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK1.
Function Double-strand-break repair protein rad21 homolog: As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions. The cohesin complex may also play a role in spindle pole assembly during mitosis. In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome. May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter. May play a role in embryonic gut development, possibly through the regulation of enteric neuron development. 64-kDa C-terminal product: May promote apoptosis.
Protein Name Double-Strand-Break Repair Protein Rad21 Homolog
Hhr21
Nuclear Matrix Protein 1
Nxp-1
Scc1 Homolog Cleaved Into - 64-Kda C-Terminal Product
64-Kda Carboxy-Terminal Product
65-Kda Carboxy-Terminal Product
Database Links Reactome: R-HSA-1221632
Reactome: R-HSA-2467813
Reactome: R-HSA-2468052
Reactome: R-HSA-2470946
Reactome: R-HSA-2500257
Reactome: R-HSA-3108214
Reactome: R-HSA-9018519
Cellular Localisation Double-Strand-Break Repair Protein Rad21 Homolog: Nucleus
Nucleus Matrix
Chromosome
Centromere
Cytoplasm
Cytoskeleton
Spindle Pole
Associates With Chromatin
Before Prophase
Scattered Along Chromosome Arms
During Prophase And Prometaphase
Most Cohesins Dissociate From The Arms Of Condensing Chromosome
Possibly Through Plk1-Mediated Phosphorylation
A Small Amount Of Cohesin Remains In Centromeric Regions And Is Removed From Chromosomes Only At The Onset Of Anaphase
At Anaphase
Cleavage By Separase/Espl1 Leads To The Dissociation Of Cohesin From Chromosomes And Chromosome Separation
64-Kda C-Terminal Product: Cytoplasm
Cytosol
Nucleus
Alternative Antibody Names Anti-Double-Strand-Break Repair Protein Rad21 Homolog antibody
Anti-Hhr21 antibody
Anti-Nuclear Matrix Protein 1 antibody
Anti-Nxp-1 antibody
Anti-Scc1 Homolog Cleaved Into - 64-Kda C-Terminal Product antibody
Anti-64-Kda Carboxy-Terminal Product antibody
Anti-65-Kda Carboxy-Terminal Product antibody
Anti-RAD21 antibody
Anti-HR21 antibody
Anti-KIAA0078 antibody
Anti-NXP1 antibody
Anti-SCC1 antibody

Information sourced from Uniprot.org

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