| Host: | Rabbit |
| Applications: | ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RAD1 |
| Gene ID: | 5810 |
| Uniprot ID: | RAD1_HUMAN |
| Immunogen Region: | 1-282 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human RAD1 (NP_002844.1). |
| Immunogen Sequence: | MPLLTQQIQDEDDQYSLVAS LDNVRNLSTILKAIHFREHA TCFATKNGIKVTVENAKCVQ ANAFIQAGIFQEFKVQEESV TFRINLTVLLDCLSIFGSSP MPGTLTALRMCYQGYGYPLM LFLEEGGVVTVCKINTQEPE ETLDFDFCSTNVINKIILQS EGLREAFSELDMTSEVLQIT MSPDKPYFRLSTFGNAGSSH LDYPKDSDLMEAFHCNQTQV NRYKISLLKPSTKALVLSC |
| Tissue Specificity | Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level). |
| Function | Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds.endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths.and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. |
| Protein Name | Cell Cycle Checkpoint Protein Rad1Hrad1Rad1-Like Dna Damage Checkpoint Protein |
| Database Links | Reactome: R-HSA-176187Reactome: R-HSA-5685938Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-9709570 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Cell Cycle Checkpoint Protein Rad1 antibodyAnti-Hrad1 antibodyAnti-Rad1-Like Dna Damage Checkpoint Protein antibodyAnti-RAD1 antibodyAnti-REC1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and ESD knockout (KO) 293T cells, using [KO Validated] ESD Rabbit polyclonal antibody (STJ11100739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89677/358170/STJ11100739_1__87167.1713122476.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ESD knockout (KO) 293T cells, using [KO Validated] ESD Rabbit polyclonal antibody (STJ11100739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")