• Western blot analysis of extracts of various cell lines, using PNMT antibody (STJ27499) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PNMT antibody (1-282) (STJ27499)

SKU:
STJ27499

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PNMT (1-282) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PNMT
Gene ID: 5409
Uniprot ID: PNMT_HUMAN
Immunogen Region: 1-282
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human PNMT (NP_002677.1).
Immunogen Sequence: MSGADRSPNAGAAPDSAPGQ AAVASAYQRFEPRAYLRNNY APPRGDLCNPNGVGPWKLRC LAQTFATGEVSGRTLIDIGS GPTVYQLLSACSHFEDITMT DFLEVNRQELGRWLQEEPGA FNWSMYSQHACLIEGKGECW QDKERQLRARVKRVLPIDVH QPQPLGAGSPAPLPADALVS AFCLEAVSPDLASFQRALDH ITTLLRPGGHLLLIGALEES WYLAGEARLTVVPVSEEEV
Function Catalyzes the transmethylation of nonepinephrine (noradrenaline) to form epinephrine (adrenaline), using S-adenosyl-L-methionine as the methyl donor. Other substrates include phenylethanolamine and octopamine. Also methylates normetanephrine.
Protein Name Phenylethanolamine N-Methyltransferase
Pnmtase
Noradrenaline N-Methyltransferase
Database Links Reactome: R-HSA-209905
Alternative Antibody Names Anti-Phenylethanolamine N-Methyltransferase antibody
Anti-Pnmtase antibody
Anti-Noradrenaline N-Methyltransferase antibody
Anti-PNMT antibody
Anti-PENT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance