• Immunohistochemistry analysis of paraffin-embedded mouse lung using ESD antibody (STJ27984) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human trachea using ESD antibody (STJ27984) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ESD antibody (STJ27984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ESD antibody (1-282) (STJ27984)

SKU:
STJ27984

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ESD (1-282) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ESD
Gene ID: 2098
Uniprot ID: ESTD_HUMAN
Immunogen Region: 1-282
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human ESD (NP_001975.1).
Immunogen Sequence: MALKQISSNKCFGGLQKVFE HDSVELNCKMKFAVYLPPKA ETGKCPALYWLSGLTCTEQN FISKSGYHQSASEHGLVVIA PDTSPRGCNIKGEDESWDFG TGAGFYVDATEDPWKTNYRM YSYVTEELPQLINANFPVDP QRMSIFGHSMGGHGALICAL KNPGKYKSVSAFAPICNPVL CPWGKKAFSGYLGTDQSKWK AYDATHLVKSYPGSQLDILI DQGKDDQFLLDGQLLPDNF
Function Serine hydrolase involved in the detoxification of formaldehyde.
Protein Name S-Formylglutathione Hydrolase
Fgh
Esterase D
Methylumbelliferyl-Acetate Deacetylase
Database Links Reactome: R-HSA-156590
Cellular Localisation Cytoplasm
Cytoplasmic Vesicle
Alternative Antibody Names Anti-S-Formylglutathione Hydrolase antibody
Anti-Fgh antibody
Anti-Esterase D antibody
Anti-Methylumbelliferyl-Acetate Deacetylase antibody
Anti-ESD antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance