• Immunohistochemistry analysis of paraffin-embedded mouse stomach using RAC2 antibody (STJ25274) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophagus using RAC2 antibody (STJ25274) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using RAC2 antibody (STJ25274) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using RAC2 Rabbit polyclonal antibody (STJ25274) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-RAC2 antibody (1-192) (STJ25274)

SKU:
STJ25274

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAC2 (1-192) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAC2
Gene ID: 5880
Uniprot ID: RAC2_HUMAN
Immunogen Region: 1-192
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-192 of human RAC2 (NP_002863.1).
Immunogen Sequence: MQAIKCVVVGDGAVGKTCLL ISYTTNAFPGEYIPTVFDNY SANVMVDSKPVNLGLWDTAG QEDYDRLRPLSYPQTDVFLI CFSLVSPASYENVRAKWFPE VRHHCPSTPIILVGTKLDLR DDKDTIEKLKEKKLAPITYP QGLALAKEIDSVKYLECSAL TQRGLKTVFDEAIRAVLCPQ PTRQQKRACSLL
Tissue Specificity Hematopoietic specific.
Post Translational Modifications (Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly.
Function Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.
Protein Name Ras-Related C3 Botulinum Toxin Substrate 2
Gx
Small G Protein
P21-Rac2
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-1222556
Reactome: R-HSA-1257604
Reactome: R-HSA-2219530
Reactome: R-HSA-4086400
Reactome: R-HSA-5668599
Reactome: R-HSA-6811558
Reactome: R-HSA-9013404
Cellular Localisation Cytoplasm
Membrane-Associated When Activated
Alternative Antibody Names Anti-Ras-Related C3 Botulinum Toxin Substrate 2 antibody
Anti-Gx antibody
Anti-Small G Protein antibody
Anti-P21-Rac2 antibody
Anti-RAC2 antibody

Information sourced from Uniprot.org

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