• Flow cytometry: K562 cells were stained with Rabbit IgG isotype control (STJ11105763, 10 Mu g/mL, blue line) followed by goat anti-Rabbit polyclonal antibody APC (1:600 dilution) staining. Non-fluorescently stained K562 cells was used as blank control (red line).
  • Flow cytometry: Daudi cells were stained with Rabbit IgG isotype control (STJ11105763, 10 Mu g/mL, blue line) followed by goat anti-Rabbit polyclonal antibody APC (1:600 dilution) staining. Non-fluorescently stained Daudi cells was used as blank control (red line).
  • Chromatin immunoprecipitation was performed with 15 Mu g of cross-linked chromatin from HeLa cells transfected with a RXRB expression vector containing a single C-terminal flag-Tag, using 3 Mu g of Rabbit IgG isotype control (STJ11105763) and RXRB Rabbit monoclonal antibody (A25648). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.
  • Immunofluorescence analysis of PC-12 cells using Rabbit IgG isotype control (STJ11105763) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Rabbit IgG isotype control (STJ11105763) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using Rabbit IgG isotype control (STJ11105763) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Rabbit IgG isotype control in paraffin-embedded rat kidney using Rabbit IgG isotype control (STJ11105763) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Rabbit IgG isotype control in paraffin-embedded mouse kidney using Rabbit IgG isotype control (STJ11105763) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Rabbit IgG isotype control in paraffin-embedded human tonsil using Rabbit IgG isotype control (STJ11105763) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Rabbit IgG isotype control antibody [S5763RM] (STJ11105763)

SKU:
STJ11105763

£109.50 - £197.50
Current Stock:
Host: Rabbit
Applications: IHC/IF/FC/IP
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Rabbit IgG isotype control is suitable for use in Immunohistochemistry, Immunofluorescence, Flow Cytometry and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S5763RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Protein A/G purification
Dilution Range: IHC-P 1:50-1:200
IF/ICC 1:50-1:200
FC 1:50-1:200
IP 0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsChIP 3 Mu g antibody for 10 Mu g-15 Mu g of Chromatin
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: None
Background The protein encoded by this gene is a transcriptional regulator and tumor suppressor, serving as an activator of genes involved in both innate and acquired immune responses.

Information sourced from Uniprot.org

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