• Confocal imaging of NIH/3T3 cells using RAB9A Rabbit monoclonal antibody (STJ11102186, dilution 1:100) (Green). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit monoclonal antibody ( dilution 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 60x.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using RAB9A Rabbit monoclonal antibody (STJ11102186) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using RAB9A Rabbit monoclonal antibody (STJ11102186) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RAB9A antibody (1-100) [S6MR] (STJ11102186)

SKU:
STJ11102186

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-RAB9A (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB9A
Gene ID: 9367
Uniprot ID: RAB9A_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RAB9A (NP_004242.1).
Immunogen Sequence: MAGKSSLFKVILLGDGGVGK SSLMNRYVTNKFDTQLFHTI GVEFLNKDLEVDGHFVTMQI WDTAGQERFRSLRTPFYRGS DCCLLTFSVDDSQSFQNLSN
Function Involved in the transport of proteins between the endosomes and the trans Golgi network. Involved in the recruitment of SGSM2 to melanosomes and is required for the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes.
Protein Name Ras-Related Protein Rab-9a
Database Links Reactome: R-HSA-6811440
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Reactome: R-HSA-9706019
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Endoplasmic Reticulum Membrane
Golgi Apparatus Membrane
Late Endosome
Cytoplasmic Vesicle
Phagosome Membrane
Phagosome
Cytoplasmic Vesicle Membrane
Melanosome
Colocalizes With Osbpl1a At The Late Endosome
Recruited To Phagosomes Containing S
Aureus Or M
Tuberculosis
Alternative Antibody Names Anti-Ras-Related Protein Rab-9a antibody
Anti-RAB9A antibody
Anti-RAB9 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance