• Western blot analysis of extracts of various cell lines, using RAB7B antibody (STJ119868) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunofluorescence analysis of HeLa cells using RAB7B rabbit polyclonal antibody (STJ119868) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-RAB7B antibody (1-199) (STJ119868)

SKU:
STJ119868

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAB7B (1-199) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB7B
Gene ID: 338382
Uniprot ID: RAB7B_HUMAN
Immunogen Region: 1-199
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human RAB7B (NP_001157994.1).
Immunogen Sequence: MNPRKKVDLKLIIVGAIGVG KTSLLHQYVHKTFYEEYQTT LGASILSKIIILGDTTLKLQ IWDTGGQERFRSMVSTFYKG SDGCILAFDVTDLESFEALD IWRGDVLAKIVPMEQSYPMV LLGNKIDLADRKVPQEVAQG WCREKDIPYFEVSAKNDINV VQAFEMLASRALSRYQSILE NHLTESIKLSPDQSRSRCC
Tissue Specificity Expressed in heart, placenta, lung, skeletal muscle and peripheral blood leukocyte.
Function Controls vesicular trafficking from endosomes to the trans-Golgi network (TGN). Acts as a negative regulator of TLR9 signaling and can suppress TLR9-triggered TNFA, IL6, and IFNB production in macrophages by promoting TLR9 lysosomal degradation. Also negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4. Promotes megakaryocytic differentiation by increasing NF-kappa-B-dependent IL6 production and subsequently enhancing the association of STAT3 with GATA1. Not involved in the regulation of the EGF- and EGFR degradation pathway.
Protein Name Ras-Related Protein Rab-7b
Database Links Reactome: R-HSA-8854214
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Late Endosome
Lysosome
Golgi Apparatus
Trans-Golgi Network
Cytoplasmic Vesicle
Phagosome
Phagosome Membrane
Lipid-Anchor
Cytoplasmic Side
Recruited To Phagosomes Containing S
Aureus Or M
Tuberculosis
Alternative Antibody Names Anti-Ras-Related Protein Rab-7b antibody
Anti-RAB7B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance