Anti-RAB5A antibody (115aa C-Term) (STJ140104)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
ImmunogenPurified recombinant peptide derived from within residues 115 aa to the C-terminus of mouse Rab5a produced in E. coli.
General Information
| Short Description | Goat polyclonal RAB5A, member RAS oncogene family (115aa C-Term) antibody for use in WB and IF in human, mouse, rat, bovine, canine, chicken, avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep and simian samples. Datashee |
| Applications | WB/IF |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 4 mg/mL |
| Purification | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range | WB 1:250-1:2000IF 1:100-1:5001. Cardoso MHS PhD Thesis NOVA University of Lisbon Portugal 2018 |
| Formulation | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Gene Symbol | RAB5A |
| Gene ID | 5868 |
| Uniprot ID | RAB5A_HUMAN |
| Accession Number | ENSG00000144566 |
| Immunogen | Purified recombinant peptide derived from within residues 115 aa to the C-terminus of mouse Rab5a produced in E. coli. |
| Immunogen Region | 115aa C-Term |
| Immunogen Sequence | ELQRQASPNIVIALSGNKAD LQRQASPNIVIALSGNKADL QRQASPNIVIALSGNKADLA RQASPNIVIALSGNKADLAN QASPNIVIALSGNKADLANK |
| Specificity | Detects Rab5a protein by Western blot in cell lysates and transfected cells with GFP-Rab5a. This Ab is specific for Rab5a; it does not recognize Rab5b and Rab5c. |
Additional Info
| Post Translational Modifications | Phosphorylation of Ser-84 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including RAB GDP dissociation inhibitors GDI1 and GDI2. (Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3. |
| Protein Name | Ras-Related Protein Rab-5a |
| Database Links | Reactome: R-HSA-1660499Reactome: R-HSA-8854214Reactome: R-HSA-8856828Reactome: R-HSA-8873719Reactome: R-HSA-8876198Reactome: R-HSA-9636383Reactome: R-HSA-9820960Reactome: R-HSA-983231 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideEarly Endosome MembraneMelanosomeCytoplasmic VesicleCell ProjectionRuffleMembraneCytoplasmCytosolPhagosome MembraneEndosome MembraneEnriched In Stage I MelanosomesAlternates Between Membrane-Bound And Cytosolic Forms (Probable) |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-5a antibodyAnti-RAB5A antibodyAnti-RAB5 antibody |
Information sourced from Uniprot.org