• Anti-Rab5a antibody at 1:500 dilution; lysates at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Immunofluorescence – anti-Rab5a antibody using NIH3T3 cells transduced with GFP-Rab5a; cells were fixed with methanol and anti-Rab5a at 1:100
  • Immunofluorescence – anti-Rab5a antibody using NIH3T3 cells transduced with GFP-Rab5a; cells were fixed with methanol and anti-Rab5a at 1:100

Anti-RAB5A antibody (115aa C-Term) (STJ140104)

SKU:
STJ140104

£100.50 - £393.50
Current Stock:
Host: Goat
Applications: WB/IF
Reactivity: Human/Rat/Mouse/Monkey/Canine
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-RAB5A, member RAS oncogene family (115aa C-Term) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 4 mg/ml
Dilution Range: WB 1:250-1:2000
IF 1:100-1:500
1. Cardoso MHS PhD Thesis NOVA University of Lisbon Portugal 2018
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Gene Symbol: RAB5A
Gene ID: 5868
Uniprot ID: RAB5A_HUMAN
Immunogen Region: 115aa C-Term
Accession Number: ENSG00000144566
Specificity: Detects Rab5a protein by Western blot in cell lysates and transfected cells with GFP-Rab5a. This Ab is specific for Rab5a; it does not recognize Rab5b and Rab5c.
Immunogen: Purified recombinant peptide derived from within residues 115 aa to the C-terminus of mouse Rab5a produced in E. coli.
Post Translational Modifications Phosphorylation of Ser-84 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including RAB GDP dissociation inhibitors GDI1 and GDI2. (Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3.
Function Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.
Protein Name Ras-Related Protein Rab-5a
Database Links Reactome: R-HSA-1660499
Reactome: R-HSA-8854214
Reactome: R-HSA-8856828
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Reactome: R-HSA-9636383
Reactome: R-HSA-983231
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Early Endosome Membrane
Melanosome
Cytoplasmic Vesicle
Cell Projection
Ruffle
Membrane
Cytoplasm
Cytosol
Phagosome Membrane
Endosome Membrane
Enriched In Stage I Melanosomes
Alternates Between Membrane-Bound And Cytosolic Forms (Probable)
Alternative Antibody Names Anti-Ras-Related Protein Rab-5a antibody
Anti-RAB5A antibody
Anti-RAB5 antibody

Information sourced from Uniprot.org

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