Anti-RAB3A antibody (27-77 aa) (STJ195808)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human RAB3A at the amino acid range 27-77
General Information
| Short Description | Rabbit polyclonal anti-Ras-related protein Rab-3A (27-77 aa) for use in WB, ELISA and IHC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAB3A |
| Gene ID | 5864 |
| Uniprot ID | RAB3A_HUMAN |
| Immunogen | Synthesized peptide derived from the human RAB3A at the amino acid range 27-77 |
| Immunogen Region | 27-77 aa |
| Specificity | This antibody detects endogenous levels of RAB3A at Human/Mouse/Rat |
Additional Info
| Post Translational Modifications | Phosphorylation of Thr-86 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB3A plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane. Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane. Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed. Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells. Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9. Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1. Also plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT. |
| Protein Name | Ras-Related Protein Rab-3a |
| Database Links | Reactome: R-HSA-181429Reactome: R-HSA-181430Reactome: R-HSA-210500Reactome: R-HSA-212676Reactome: R-HSA-264642Reactome: R-HSA-6798695Reactome: R-HSA-8873719Reactome: R-HSA-8876198Reactome: R-HSA-888590Reactome: R-HSA-9662360 |
| Cellular Localisation | CytoplasmCytosolLysosomeCytoplasmic VesicleSecretory VesicleCell ProjectionAxonCell MembraneLipid-AnchorCytoplasmic SidePresynapsePostsynapseCycles Between A Vesicle-Associated Gtp-Bound Form And A Cytosolic Gdp-Bound Form |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-3a antibodyAnti-RAB3A antibody |
Information sourced from Uniprot.org