• Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RAB3A antibody (27-77 aa) (STJ195808)

SKU:
STJ195808

Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ras-related protein Rab-3A (27-77 aa) is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB3A
Gene ID: 5864
Uniprot ID: RAB3A_HUMAN
Immunogen Region: 27-77 aa
Specificity: This antibody detects endogenous levels of RAB3A at Human/Mouse/Rat
Immunogen: Synthesized peptide derived from the human RAB3A at the amino acid range 27-77
Post Translational Modifications Phosphorylation of Thr-86 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.
Function Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane. Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane. Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed. Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells. Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9. Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1. Also plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT. Interacts with MADD (via uDENN domain).the GTP-bound form is preferred for interaction.
Protein Name Ras-Related Protein Rab-3a
Database Links Reactome: R-HSA-181429
Reactome: R-HSA-181430
Reactome: R-HSA-210500
Reactome: R-HSA-212676
Reactome: R-HSA-264642
Reactome: R-HSA-6798695
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Reactome: R-HSA-888590
Reactome: R-HSA-9662360
Cellular Localisation Cytoplasm
Cytosol
Lysosome
Cytoplasmic Vesicle
Secretory Vesicle
Cell Projection
Axon
Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Presynapse
Postsynapse
Cycles Between A Vesicle-Associated Gtp-Bound Form And A Cytosolic Gdp-Bound Form
Alternative Antibody Names Anti-Ras-Related Protein Rab-3a antibody
Anti-RAB3A antibody

Information sourced from Uniprot.org

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