![Western blot analysis of lysates from wild type (WT) and RAB2A knockout (KO) 293T cells, using [KO Validated] RAB2A Rabbit polyclonal antibody (STJ11100858) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89792/358262/STJ11100858_1__14517.1713122602.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and RAB2A knockout (KO) 293T cells, using [KO Validated] RAB2A Rabbit polyclonal antibody (STJ11100858) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western blot analysis of lysates from wild type (WT) and RAB2A knockout (KO) 293T cells, using [KO Validated] RAB2A Rabbit polyclonal antibody (STJ11100858) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89792/358262/STJ11100858_1__14517.1713122602.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and RAB2A knockout (KO) 293T cells, using [KO Validated] RAB2A Rabbit polyclonal antibody (STJ11100858) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RAB2A |
| Gene ID: | 5862 |
| Uniprot ID: | RAB2A_HUMAN |
| Immunogen Region: | 1-212 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human RAB2A (NP_002856.1). |
| Immunogen Sequence: | MAYAYLFKYIIIGDTGVGKS CLLLQFTDKRFQPVHDLTIG VEFGARMITIDGKQIKLQIW DTAGQESFRSITRSYYRGAA GALLVYDITRRDTFNHLTTW LEDARQHSNSNMVIMLIGNK SDLESRREVKKEEGEAFARE HGLIFMETSAKTASNVEEAF INTAKEIYEKIQEGVFDINN EANGIKIGPQHAATNATHAG NQGGQQAGGGCC |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between active GTP-bound and inactive GDP-bound states. In their active state, drive transport of vesicular carriers from donor organelles to acceptor organelles to regulate the membrane traffic that maintains organelle identity and morphology. Required for protein transport from the endoplasmic reticulum to the Golgi complex. Regulates the compacted morphology of the Golgi (Probable). Together with RAB2B redundantly required for efficient autophagic flux. |
| Protein Name | Ras-Related Protein Rab-2a |
| Database Links | Reactome: R-HSA-162658 Reactome: R-HSA-8873719 |
| Cellular Localisation | Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane Lipid-Anchor Cytoplasmic Side Melanosome Endoplasmic Reticulum Membrane Golgi Apparatus Membrane Cytoplasmic Vesicle Secretory Vesicle Acrosome Autophagosome Membrane Localized In The Golgi Apparatus In The Round Spermatids And In The Acrosome In The Elongating Spermatid Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-2a antibody Anti-RAB2A antibody Anti-RAB2 antibody |
Information sourced from Uniprot.org