Host: | Goat |
Applications: | WB/IHC-F/IHC-P/IF/ELISA |
Reactivity: | Human/Rat/Mouse/Monkey/Canine |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Goat polyclonal antibody anti-RAB27, member RAS oncogene family (120aa C-Term) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS, 20% Glycerol and 0.05% Sodium Azide. |
Purification: | This antibody is epitope-affinity purified from goat antiserum. |
Concentration: | 3 mg/ml |
Dilution Range: | WB 1:500-1:2000 IHC-F 1:50-1:400 IHC-P 1:50-1:400 IF 1:50-1:250 ELISA ND Ferreira JV Soares AR Ramalho J et al. Sci Adv 2022 Mar PMID: 35333565 Ferreira JV Rosa Soares A Ramalho JS et al. PLoS One 2019 Oct. PMID: 31613922 |
Storage Instruction: | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Gene Symbol: | RAB27A |
Gene ID: | 5873 |
Uniprot ID: | RB27A_HUMAN |
Immunogen Region: | 120aa C-Term |
Accession Number: | ENSG00000069974 |
Specificity: | Detects total Rab27 levels by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab27a and GFP-Rab27b cds. |
Immunogen: | Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab27a and rab27b produced in E. coli. |
Tissue Specificity | Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells. |
Function | Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. |
Protein Name | Ras-Related Protein Rab-27a Rab-27 Gtp-Binding Protein Ram |
Database Links | Reactome: R-HSA-264876 Reactome: R-HSA-6798695 Reactome: R-HSA-8873719 Reactome: R-HSA-8876198 |
Cellular Localisation | Membrane Lipid-Anchor Melanosome Late Endosome Lysosome Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv Localizes To Endosomal Exocytic Vesicles |
Alternative Antibody Names | Anti-Ras-Related Protein Rab-27a antibody Anti-Rab-27 antibody Anti-Gtp-Binding Protein Ram antibody Anti-RAB27A antibody Anti-RAB27 antibody |
Information sourced from Uniprot.org