• Western blot analysis of extracts of various cell lines, using RAB24 antibody (STJ110692) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.
  • Immunoprecipitation analysis of 200 Mu g extracts of MCF-7 cells, using 3 Mu g RAB24 antibody (STJ110692). Western blot was performed from the immunoprecipitate using RAB24 antibody (STJ110692) at a dilution of 1:1000.
  • Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug RAB24 antibody (STJ110692). Western blot was performed from the immunoprecipitate using RAB24 antibody (STJ110692) at a dilition of 1:1000.
  • Western blot analysis of various lysates using RAB24 Rabbit polyclonal antibody (STJ110692) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.

Anti-RAB24 antibody (1-203) (STJ110692)

SKU:
STJ110692

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Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAB24 (1-203) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB24
Gene ID: 53917
Uniprot ID: RAB24_HUMAN
Immunogen Region: 1-203
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-203 of human RAB24 (NP_001026847.1).
Immunogen Sequence: MSGQRVDVKVVMLGKEYVGK TSLVERYVHDRFLVGPYQNT IGAAFVAKVMSVGDRTVTLG IWDTAGSERYEAMSRIYYRG AKAAIVCYDLTDSSSFERAK FWVKELRSLEEGCQIYLCGT KSDLLEEDRRRRRVDFHDVQ DYADNIKAQLFETSSKTGQS VDELFQKVAEDYVSVAAFQV MTEDKGVDLGQKPNPYFYSC CHH
Post Translational Modifications Isoprenylation is inefficient compared to other Rab family members.
Function May be involved in autophagy-related processes.
Protein Name Ras-Related Protein Rab-24
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-8873719
Cellular Localisation Cytoplasm
Cytosol
Membrane
Lipid-Anchor
Only About 20-25% Is Recovered In The Particulate Fraction
Alternative Antibody Names Anti-Ras-Related Protein Rab-24 antibody
Anti-RAB24 antibody

Information sourced from Uniprot.org

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