Anti-RAB24 antibody (1-203) (STJ110692)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RAB24 (1-203) antibody for use in WB, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAB24 |
| Gene ID | 53917 |
| Uniprot ID | RAB24_HUMAN |
| Immunogen Region | 1-203 |
| Immunogen Sequence | MSGQRVDVKVVMLGKEYVGK TSLVERYVHDRFLVGPYQNT IGAAFVAKVMSVGDRTVTLG IWDTAGSERYEAMSRIYYRG AKAAIVCYDLTDSSSFERAK FWVKELRSLEEGCQIYLCGT KSDLLEEDRRRRRVDFHDVQ DYADNIKAQLFETSSKTGQS VDELFQKVAEDYVSVAAFQV MTEDKGVDLGQKPNPYFYSC CHH |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-203 of human RAB24 (NP_001026847.1). |
Additional Info
| Post Translational Modifications | Prenylated.prenylation is required for RAB24 localization to autophagosomes. Isoprenylation is inefficient compared to other Rab family members. Phosphorylated at Tyr-17 and Tyr-172. Cytosolic pool of RAB24 is more phosphorylated than the membrane-associated pool. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB24 is an atypical RAB protein that presents low GTPase activity and thereby exists predominantly in the GTP-bound active state. RAB24 is required for the clearance of late autophagic vacuoles under basal conditions. It is not needed for starvation-induced autophagy. Involved in the modulation of meiotic apparatus assembly and meiotic progression during oocyte maturation, possibly through regulation of kinetochore-microtubule interaction. |
| Protein Name | Ras-Related Protein Rab-24 |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-8873719 |
| Cellular Localisation | CytoplasmCytosolMembraneLipid-AnchorCytoplasmic VesicleAutophagosome MembranePerinuclear RegionCytoskeletonSpindleOnly About 20% Is Recovered In The Particulate FractionRab24 Localizes In Perinuclear Region And In The Limiting Membranes Of Autophagic Compartments Under Basal ConditionsRab24 Is Localized In The Cytoplasm With An Accumulated Distribution In Nuclear Region At Germinal Vesicle (Gv) Stage Of Oocyte Meiotic ProgressionAt Pre-Metaphase I StageLocalized In The Cytoplasm With A Particular Concentration Around ChromosomesAs The Oocytes Enter Metaphase ILocated To The Spindle RegionSimilar Distribution Pattern Is Observed In Mii Oocytes |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-24 antibodyAnti-RAB24 antibody |
Information sourced from Uniprot.org