• Anti-Rab1a antibody at 1:1000 dilution lysates at 50 µg per lane rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Immunohistochemistry of human miocardium using anti-Rab1a antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab1a antibody at 1:250:DAB detection.
  • Immunohistochemistry of human skin using anti-Rab1a antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab1a antibody at 1:250:DAB detection.
  • Immunohistochemistry of mouse stomach using anti-Rab1a antibody and FFPE tissue after heat-induced antigen retrieval. Anti-Rab1a antibody at 1:250:DAB detection.

Anti-RAB1A antibody (107aa C-Term) (STJ140152)

SKU:
STJ140152

Current Stock:
Host: Goat
Applications: WB/IHC-F/IHC-P
Reactivity: Human/Rat/Mouse/Monkey/Canine
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-RAB1, member RAS oncogene family (107aa C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3 mg/ml
Dilution Range: WB 1:1000-1:2000
IHC-F 1:100-1:500
IHC-P 1:100-1:500
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Gene Symbol: RAB1A
Gene ID: 5861
Uniprot ID: RAB1A_HUMAN
Immunogen Region: 107aa C-Term
Accession Number: ENSG00000138069
Specificity: Detects endogenous levels of Rab1a (HUH, LS174T, MDA-MB-435, MCF7, Cos7, MCR, AtT20, IMCD3, HeLa, MDCK) by Western blot. This antibody does not cross-react with Rab1b.
Immunogen: Purified recombinant peptide derived from within residues 107 aa to the C-terminus of Rab1a produced in E. coli.
Immunogen Sequence: QEIDRYASENVNKLLVGNKC DLTTKKVVDYTTAKEFADSL GIPFLETSAKNATNVEQSFM TMAAEIKKRMGPGATAGGA
Post Translational Modifications Phosphorylated by CDK1 kinase during mitosis. (Microbial infection) Phosphocholinated at Ser-79 by L.pneumophila AnkX, leading to displace GDP dissociation inhibitors (GDI). Both GDP-bound and GTP-bound forms can be phosphocholinated. Dephosphocholinated by L.pneumophila Lem3, restoring accessibility to L.pneumophila GTPase effector LepB. (Microbial infection) Glycosylated by S.typhimurium protein Ssek3: arginine GlcNAcylation prevents GTPase activity, thereby disrupting vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment.
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Required to modulate the compacted morphology of the Golgi. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport.
Protein Name Ras-Related Protein Rab-1a
Ypt1-Related Protein
Database Links Reactome: R-HSA-162658
Reactome: R-HSA-204005
Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Golgi Apparatus
Endoplasmic Reticulum
Early Endosome
Cytoplasm
Cytosol
Membrane
Melanosome
Alternates Between Membrane-Associated And Cytosolic Forms
Alternative Antibody Names Anti-Ras-Related Protein Rab-1a antibody
Anti-Ypt1-Related Protein antibody
Anti-RAB1A antibody
Anti-RAB1 antibody

Information sourced from Uniprot.org

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