Anti-RAB11B antibody [8G4] (STJA0032575)
SPECIFICATIONS
ClonalityMonoclonal
HostRat
ConjugationUnconjugated
IsotypeIgG2a
ImmunogenRecombinant mouse RAB11B
General Information
| Short Description | Rat monoclonal anti-RAB11B for use in WB, ICC and IF in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF |
| Host | Rat |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 8G4 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200 |
| Formulation | Liquid in PBS containing 0.09% sodium azide as a preservative. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Rab11b |
| Gene ID | 19326 |
| Uniprot ID | RB11B_MOUSE |
| Immunogen | Recombinant mouse RAB11B |
Additional Info
| Tissue Specificity | Abundantly expressed in brain, heart and testis. Also detected in kidney and pancreatic islets. |
| Post Translational Modifications | Citrullinated by PADI4. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The small Rab GTPase RAB11B plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis. Promotes Rabin8/RAB3IP preciliary vesicular trafficking to mother centriole by forming a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, thereby regulating ciliogenesis initiation. On the contrary, upon LPAR1 receptor signaling pathway activation, interaction with phosphorylated WDR44 prevents Rab11-RAB3IP-RAB11FIP3 complex formation and cilia growth. |
| Protein Name | Ras-Related Protein Rab-11b |
| Database Links | Reactome: R-MMU-8854214Reactome: -MMU-8873719 |
| Cellular Localisation | Recycling Endosome MembraneLipid-AnchorCytoplasmic SideCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembranePhagosome MembraneColocalizes With Rab11afip1 On Punctate Vesicles |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-11b antibodyAnti-Rab11b antibody |
Information sourced from Uniprot.org