| Host: |
Goat |
| Applications: |
WB/IHC-F/IHC-P/IF |
| Reactivity: |
Human/Rat/Mouse/Monkey/Canine |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-RAB11, member RAS oncogene family (110aa C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: |
This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: |
3 mg/ml |
| Dilution Range: |
WB 1:250-1:2000IF 1:50-1:250IHC-P 1:100-1:400IHC-F 1:100-1:400Cardoso MHS PhD Thesis NOVA University of Lisbon Portugal 2018 |
| Storage Instruction: |
Store at-20 C for long-term storage. Store at 2-8 C for up to one month. |
| Gene Symbol: |
RAB11A |
| Gene ID: |
8766 |
| Uniprot ID: |
RB11A_HUMAN |
| Immunogen Region: |
110aa C-Term |
| Accession Number: |
ENSG00000103769 |
| Specificity: |
Detects a band of 24 kDa by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab11a cds. This antibody is specific to Rab11a. It does not recognize Rab11b. |
| Immunogen: |
Purified recombinant peptide derived from within residues 110 aa to the C-terminus of mouse Rab11a produced in E. coli. |
| Post Translational Modifications | (Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The small Rab GTPase RAB11A regulates endocytic recycling. Forms a functional Rab11/FIP3/dynein complex that regulates the movement of peripheral sorting endosomes (SE) along microtubule tracks toward the microtubule organizing center/centrosome, generating the endosomal recycling compartment (ERC). Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes. Promotes Rabin8/RAB3IP preciliary vesicular trafficking to mother centriole by forming a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, thereby regulating ciliogenesis initiation. On the contrary, upon LPAR1 receptor signaling pathway activation, interaction with phosphorylated WDR44 prevents Rab11-RAB3IP-RAB11FIP3 complex formation and cilia growth. Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-endososomal dependent export route via interaction with WDR44. |
| Protein Name | Ras-Related Protein Rab-11aRab-11Yl8 |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-432040Reactome: R-HSA-5620912Reactome: R-HSA-5620916Reactome: R-HSA-8854214Reactome: R-HSA-8873719 |
| Cellular Localisation | Cell MembraneLipid-AnchorEndosome MembraneRecycling Endosome MembraneCleavage FurrowCytoplasmic VesiclePhagosomeCytoplasmic Vesicle MembraneGolgi ApparatusTrans-Golgi NetworkLocalized To Wdr44-Positive Endosomes And TubulesTranslocates With Rab11fip2 From The Vesicles Of The Endocytic Recycling Compartment (Erc) To The Plasma MembraneDuring InterphaseLocalized In Vesicles Continuously Moving From Peripheral Sorting Endosomes Towards The Pericentrosomal ErcLocalizes To The Cleavage FurrowColocalizes With Pard3PrkciExoc5OclnPodxl And Rab8a In Apical Membrane Initiation Sites (Amis) During The Generation Of Apical Surface And LumenogenesisRecruited To Phagosomes Containing SAureus Or MTuberculosisLocalized To Rhodopsin Transport Carriers When Interacting With Rab11afip3 And Asap1 In Photoreceptors |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-11a antibodyAnti-Rab-11 antibodyAnti-Yl8 antibodyAnti-RAB11A antibodyAnti-RAB11 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
