• Anti-Rab11a antibody Immunohistochemistry staining of human mammary tissue; immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval; antibody concentration 1:200
  • Anti-Rab11a antibody at 1:2500 dilution; lysates at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Immunofluorescence – anti-Rab11a antibody at 1:100 dilution in NHI:3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin

Anti-RAB11A antibody (110aa C-Term) (STJ140068)

SKU:
STJ140068

Current Stock:
Host: Goat
Applications: WB/IHC-F/IHC-P/IF
Reactivity: Human/Rat/Mouse/Monkey/Canine
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-RAB11, member RAS oncogene family (110aa C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3 mg/ml
Dilution Range: WB 1:250-1:2000
IF 1:50-1:250
IHC-P 1:100-1:400
IHC-F 1:100-1:400
Cardoso MHS PhD Thesis NOVA University of Lisbon Portugal 2018
Storage Instruction: Store at-20 C for long-term storage. Store at 2-8 C for up to one month.
Gene Symbol: RAB11A
Gene ID: 8766
Uniprot ID: RB11A_HUMAN
Immunogen Region: 110aa C-Term
Accession Number: ENSG00000103769
Specificity: Detects a band of 24 kDa by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab11a cds. This antibody is specific to Rab11a. It does not recognize Rab11b.
Immunogen: Purified recombinant peptide derived from within residues 110 aa to the C-terminus of mouse Rab11a produced in E. coli.
Post Translational Modifications (Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3.
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The small Rab GTPase RAB11A regulates endocytic recycling. Forms a functional Rab11/FIP3/dynein complex that regulates the movement of peripheral sorting endosomes (SE) along microtubule tracks toward the microtubule organizing center/centrosome, generating the endosomal recycling compartment (ERC). Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes. Promotes Rabin8/RAB3IP preciliary vesicular trafficking to mother centriole by forming a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, thereby regulating ciliogenesis initiation. On the contrary, upon LPAR1 receptor signaling pathway activation, interaction with phosphorylated WDR44 prevents Rab11-RAB3IP-RAB11FIP3 complex formation and cilia growth. Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-endososomal dependent export route via interaction with WDR44.
Protein Name Ras-Related Protein Rab-11a
Rab-11
Yl8
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-432040
Reactome: R-HSA-5620912
Reactome: R-HSA-5620916
Reactome: R-HSA-8854214
Reactome: R-HSA-8873719
Cellular Localisation Cell Membrane
Lipid-Anchor
Endosome Membrane
Recycling Endosome Membrane
Cleavage Furrow
Cytoplasmic Vesicle
Phagosome
Cytoplasmic Vesicle Membrane
Golgi Apparatus
Trans-Golgi Network
Localized To Wdr44-Positive Endosomes And Tubules
Translocates With Rab11fip2 From The Vesicles Of The Endocytic Recycling Compartment (Erc) To The Plasma Membrane
During Interphase
Localized In Vesicles Continuously Moving From Peripheral Sorting Endosomes Towards The Pericentrosomal Erc
Localizes To The Cleavage Furrow
Colocalizes With Pard3
Prkci
Exoc5
Ocln
Podxl And Rab8a In Apical Membrane Initiation Sites (Amis) During The Generation Of Apical Surface And Lumenogenesis
Recruited To Phagosomes Containing S
Aureus Or M
Tuberculosis
Localized To Rhodopsin Transport Carriers When Interacting With Rab11afip3 And Asap1 In Photoreceptors
Alternative Antibody Names Anti-Ras-Related Protein Rab-11a antibody
Anti-Rab-11 antibody
Anti-Yl8 antibody
Anti-RAB11A antibody
Anti-RAB11 antibody

Information sourced from Uniprot.org

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