| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RAB10 (98-200) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RAB10 |
| Gene ID: |
10890 |
| Uniprot ID: |
RAB10_HUMAN |
| Immunogen Region: |
98-200 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 98-200 of human RAB10 (NP_057215.3). |
| Immunogen Sequence: |
ENISKWLRNIDEHANEDVER MLLGNKCDMDDKRVVPKGKG EQIAREHGIRFFETSAKANI NIEKAFLTLAEDILRKTPVK EPNSENVDISSGGGVTGWKS K |
| Tissue Specificity | Expressed in the hippocampus. Expressed in neutrophils (at protein level). Expressed in the testis (at protein level). |
| Post Translational Modifications | Ubiquitinated upon Legionella pneumophila infection. Ubiquitination does not lead to proteasomal degradation. Phosphorylation of Thr-73 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Also plays a specific role in asymmetric protein transport to the plasma membrane. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane. In epithelial cells, it regulates transport from the Golgi to the basolateral membrane. May play a role in the basolateral recycling pathway and in phagosome maturation. May play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion. Together with LRRK2, RAB8A, and RILPL1, it regulates ciliogenesis. When phosphorylated by LRRK2 on Thr-73, binds RILPL1 and inhibits ciliogenesis. (Microbial infection) Upon Legionella pneumophila infection promotes endoplasmic reticulum recruitment and bacterial replication. Plays a role in remodeling the Legionella-containing vacuole (LCV) into an endoplasmic reticulum-like vacuole. |
| Protein Name | Ras-Related Protein Rab-10 |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-6798695Reactome: R-HSA-8873719Reactome: R-HSA-8876198 |
| Cellular Localisation | Cytoplasmic Vesicle MembraneLipid-AnchorCytoplasmic SideGolgi Apparatus MembraneGolgi ApparatusTrans-Golgi Network MembraneEndosome MembraneRecycling Endosome MembraneCytoplasmic VesiclePhagosome MembraneCytoplasmCytoskeletonCilium Basal BodyEndoplasmic Reticulum MembranePerinuclear RegionAssociates With Slc2a4/Glut4 Storage VesiclesLocalizes To The Base Of The Cilium When Phosphorylated By Lrrk2 On Thr-73Transiently Associates With PhagosomesLocalizes To The Endoplasmic Reticulum At Domains Of New Tubule Growth |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-10 antibodyAnti-RAB10 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
