• Western blot analysis of lysates from Mouse liver, using PYCRL Rabbit polyclonal antibody (STJ119800) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PYCRL antibody (13-286) (STJ119800)

SKU:
STJ119800

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PYCRL (13-286) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PYCR3
Gene ID: 65263
Uniprot ID: P5CR3_HUMAN
Immunogen Region: 13-286
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 13-286 of human PYCRL (NP_075566.2).
Immunogen Sequence: MAAAEPSPRRVGFVGAGRMA GAIAQGLIRAGKVEAQHILA SAPTDRNLCHFQALGCRTTH SNQEVLQSCLLVIFATKPHV LPAVLAEVAPVVTTEHILVS VAAGVSLSTLEELLPPNTRV LRVLPNLPCVVQEGAIVMAR GRHVGSSETKLLQHLLEACG RCEEVPEAYVDIHTGLSGSG VAFVCAFSEALAEGAVKMGM PSSLAHRIAAQTLLGTAKML LHEGQHPAQLRSDVCTPGG
Function Enzyme that catalyzes the last step in proline biosynthesis. Proline is synthesized from either glutamate or ornithine.both are converted to pyrroline-5-carboxylate (P5C), and then to proline via pyrroline-5-carboxylate reductases (PYCRs). PYCRL is exclusively linked to the conversion of ornithine to proline.
Protein Name Pyrroline-5-Carboxylate Reductase 3
P5c Reductase 3
P5cr 3
Pyrroline-5-Carboxylate Reductase-Like Protein
Database Links Reactome: R-HSA-8964539
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Pyrroline-5-Carboxylate Reductase 3 antibody
Anti-P5c Reductase 3 antibody
Anti-P5cr 3 antibody
Anti-Pyrroline-5-Carboxylate Reductase-Like Protein antibody
Anti-PYCR3 antibody
Anti-PYCRL antibody

Information sourced from Uniprot.org

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