• Immunofluorescence analysis of L929 cells using PYCR2 Rabbit polyclonal antibody (STJ117349) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using PYCR2 Rabbit polyclonal antibody (STJ117349) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PYCR2 Rabbit polyclonal antibody (STJ117349) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PYCR2 in paraffin-embedded Human liver cancer using PYCR2 Rabbit polyclonal antibody (STJ117349) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PYCR2 in paraffin-embedded Rat liver using PYCR2 Rabbit polyclonal antibody (STJ117349) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PYCR2 in paraffin-embedded Mouse kidney using PYCR2 Rabbit polyclonal antibody (STJ117349) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using PYCR2 Rabbit polyclonal antibody (STJ117349) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PYCR2 antibody (271-320) (STJ117349)

SKU:
STJ117349

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PYCR2 (271-320) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PYCR2
Gene ID: 29920
Uniprot ID: P5CR2_HUMAN
Immunogen Region: 271-320
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 271-320 of human PYCR2 (NP_037460.2).
Immunogen Sequence: MADQEKISPAALKKTLLDRV KLESPTVSTLTPSSPGKLLT RSLALGGKKD
Tissue Specificity Detected in erythrocytes (at protein level). Expressed in fetal brain.
Function Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH. Involved in cellular response to oxidative stress.
Protein Name Pyrroline-5-Carboxylate Reductase 2
P5c Reductase 2
P5cr 2
Database Links Reactome: R-HSA-8964539
Cellular Localisation Cytoplasm
Mitochondrion
Alternative Antibody Names Anti-Pyrroline-5-Carboxylate Reductase 2 antibody
Anti-P5c Reductase 2 antibody
Anti-P5cr 2 antibody
Anti-PYCR2 antibody

Information sourced from Uniprot.org

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