• Western blot analysis of lysates from wild type (WT) and PTPN1 knockout (KO) HeLa (KO) cells, using [KO Validated] PTPN1 Rabbit polyclonal antibody (STJ11104770) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of lysates from C6 cells, using [KO Validated] PTPN1 Rabbit polyclonal antibody (STJ11104770) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of lysates from HeLa cells, using [KO Validated] PTPN1 Rabbit polyclonal antibody (STJ11104770) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PTPN1 antibody (281-435) (STJ11104770)

SKU:
STJ11104770

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Tyrosine-Protein Phosphatase Non-Receptor Type 1 (281-435) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTPN1
Gene ID: 5770
Uniprot ID: PTN1_HUMAN
Immunogen Region: 281-435
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 281-435 of human PTPN1 (NP_002818.1).
Immunogen Sequence: IMGDSSVQDQWKELSHEDLE PPPEHIPPPPRPPKRILEPH NGKCREFFPNHQWVKEETQE DKDCPIKEEKGSPLNAAPYG IESMSQDTEVRSRVVGGSLR GAQAASPAKGEPSLPEKDED HALSYWKPFLVNMCVATVLT AGAYLCYRFLFNSNT
Tissue Specificity Expressed in keratinocytes (at protein level).
Post Translational Modifications Oxidized on Cys-215.the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50. S-nitrosylation of Cys-215 inactivates the enzyme activity. Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.
Function Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK.inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
Protein Name Tyrosine-Protein Phosphatase Non-Receptor Type 1
Protein-Tyrosine Phosphatase 1b
Ptp-1b
Database Links Reactome: R-HSA-354192
Reactome: R-HSA-6807004
Reactome: R-HSA-77387
Reactome: R-HSA-877312
Reactome: R-HSA-8849472
Reactome: R-HSA-9022699
Reactome: R-HSA-912694
Reactome: R-HSA-982772
Cellular Localisation Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Interacts With Epha3 At The Cell Membrane
Alternative Antibody Names Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 1 antibody
Anti-Protein-Tyrosine Phosphatase 1b antibody
Anti-Ptp-1b antibody
Anti-PTPN1 antibody
Anti-PTP1B antibody

Information sourced from Uniprot.org

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