• Western blot analysis of extracts of various cell lines, using PTCRA antibody (STJ29230) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PTCRA antibody (172-281) (STJ29230)

SKU:
STJ29230

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PTCRA (172-281) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTCRA
Gene ID: 171558
Uniprot ID: PTCRA_HUMAN
Immunogen Region: 172-281
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 172-281 of human PTCRA (NP_612153.2).
Immunogen Sequence: LCDPAGPLPSPATTTRLRAL GSHRLHPATETGGREATSSP RPQPRDRRWGDTPPGRKPGS PVWGEGSYLSSYPTCPAQAW CSRSALRAPSSSLGAFFAGD LPPPLQAGAA
Tissue Specificity Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.
Function The pre-T-cell receptor complex (composed of PTCRA, TCRB and the CD3 complex) regulates early T-cell development.
Protein Name Pre T-Cell Antigen Receptor Alpha
Pt-Alpha
Pta
Pt-Alpha-Tcr
Database Links Reactome: R-HSA-9013508
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Pre T-Cell Antigen Receptor Alpha antibody
Anti-Pt-Alpha antibody
Anti-Pta antibody
Anti-Pt-Alpha-Tcr antibody
Anti-PTCRA antibody

Information sourced from Uniprot.org

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