• Immunohistochemistry analysis of PSMG1 in paraffin-embedded mouse spleen using PSMG1 Rabbit polyclonal antibody (STJ117534) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PSMG1 in paraffin-embedded human appendix using PSMG1 Rabbit polyclonal antibody (STJ117534) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PSMG1 in paraffin-embedded rat spleen using PSMG1 Rabbit polyclonal antibody (STJ117534) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HeLa cells, using PSMG1 Rabbit polyclonal antibody (STJ117534) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.

Anti-PSMG1 antibody (1-288) (STJ117534)

SKU:
STJ117534

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PSMG1 (1-288) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PSMG1
Gene ID: 8624
Uniprot ID: PSMG1_HUMAN
Immunogen Region: 1-288
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-288 of human PSMG1 (NP_003711.1).
Immunogen Sequence: MAATFFGEVVKAPCRAGTED EEEEEEGRRETPEDREVRLQ LARKREVRLLRRQTKTSLEV SLLEKYPCSKFIIAIGNNAV AFLSSFVMNSGVWEEVGCAK LWNEWCRTTDTTHLSSTEAF CVFYHLKSNPSVFLCQCSCY VAEDQQYQWLEKVFGSCPRK NMQITILTCRHVTDYKTSES TGSLPSPFLRALKTQNFKDS ACCPLLEQPNIVHDLPAAVL SYCQVWKIPAILYLCYTDV
Tissue Specificity In the adult, detected in brain, colon, leukocytes, breast and testis. Widely expressed in the fetus. Also expressed in a variety of proliferating cell lines.
Post Translational Modifications Degraded by the proteasome upon completion of 20S proteasome maturation.
Function Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG2. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.
Protein Name Proteasome Assembly Chaperone 1
Pac-1
Chromosome 21 Leucine-Rich Protein
C21-Lrp
Down Syndrome Critical Region Protein 2
Cellular Localisation Cytoplasm
Endoplasmic Reticulum
Alternative Antibody Names Anti-Proteasome Assembly Chaperone 1 antibody
Anti-Pac-1 antibody
Anti-Chromosome 21 Leucine-Rich Protein antibody
Anti-C21-Lrp antibody
Anti-Down Syndrome Critical Region Protein 2 antibody
Anti-PSMG1 antibody
Anti-C21LRP antibody
Anti-DSCR2 antibody
Anti-PAC1 antibody

Information sourced from Uniprot.org

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