Anti-PRPF8 antibody (2200-2335) [S7MR] (STJ11102107)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal PRPF8 (2200-2335) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S7MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PRPF8 |
| Gene ID | 10594 |
| Uniprot ID | PRP8_HUMAN |
| Immunogen Region | 2200-2335 |
| Immunogen Sequence | MADNPSWDGEKTIIITCSFT PGSCTLTAYKLTPSGYEWGR QNTDKGNNPKGYLPSHYERV QMLLSDRFLGFFMVPAQSSW NYNFMGVRHDPNMKYELQLA NPKEFYHEVHRPSHFLNFAL LQEGEVYSADREDLYA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 2200-2335 of human PRPF8 (Q6P2Q9). |
Additional Info
| Tissue Specificity | Widely expressed. |
| Function | Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as a scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site. |
| Protein Name | Pre-Mrna-Processing-Splicing Factor 8220 Kda U5 Snrnp-Specific ProteinPrp8 HomologSplicing Factor Prp8P220 |
| Database Links | Reactome: R-HSA-72163Reactome: R-HSA-72165 |
| Cellular Localisation | NucleusNucleus Speckle |
| Alternative Antibody Names | Anti-Pre-Mrna-Processing-Splicing Factor 8 antibodyAnti-220 Kda U5 Snrnp-Specific Protein antibodyAnti-Prp8 Homolog antibodyAnti-Splicing Factor Prp8 antibodyAnti-P220 antibodyAnti-PRPF8 antibodyAnti-PRPC8 antibody |
Information sourced from Uniprot.org