• Immunohistochemistry analysis of paraffin-embedded mouse brain using PRPF31 antibody (STJ28299) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human gastric cancer using PRPF31 antibody (STJ28299) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using PRPF31 antibody (STJ28299) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PRPF31 antibody (STJ28299) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PRPF31 antibody (1-200) (STJ28299)

SKU:
STJ28299

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRPF31 (1-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRPF31
Gene ID: 26121
Uniprot ID: PRP31_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PRPF31 (NP_056444.3).
Immunogen Sequence: MSLADELLADLEEAAEEEEG GSYGEEEEEPAIEDVQEETQ LDLSGDSVKTIAKLWDSKMF AEIMMKIEEYISKQAKASEV MGPVEAAPEYRVIVDANNLT VEIENELNIIHKFIRDKYSK RFPELESLVPNALDYIRTVK ELGNSLDKCKNNENLQQILT NATIMVVSVTASTTQGQQLS EEELERLEEACDMALELNAS
Tissue Specificity Ubiquitously expressed.
Function Involved in pre-mRNA splicing as component of the spliceosome. Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.
Protein Name U4/U6 Small Nuclear Ribonucleoprotein Prp31
Pre-Mrna-Processing Factor 31
Serologically Defined Breast Cancer Antigen Ny-Br-99
U4/U6 Snrnp 61 Kda Protein
Protein 61k
Hprp31
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Nucleus Speckle
Cajal Body
Predominantly Found In Speckles And In Cajal Bodies
Alternative Antibody Names Anti-U4/U6 Small Nuclear Ribonucleoprotein Prp31 antibody
Anti-Pre-Mrna-Processing Factor 31 antibody
Anti-Serologically Defined Breast Cancer Antigen Ny-Br-99 antibody
Anti-U4/U6 Snrnp 61 Kda Protein antibody
Anti-Protein 61k antibody
Anti-Hprp31 antibody
Anti-PRPF31 antibody
Anti-PRP31 antibody

Information sourced from Uniprot.org

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