Anti-proteinase 3/myeloblastin antibody (88-98) (STJ73068)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-proteinase 3/myeloblastin (88-98) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications. |
| Applications | Pep-ELISA/WB/IHC/IF/FC |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 27kDa band observed in Human Spleen lysates, approx. 25kDa in Human Bone Marrow lysates, and approx. 26kDa in preliminary testing of THP-1 cell lysate (calculated MW of 27.8kDa |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | PRTN3 |
| Gene ID | 5657 |
| Uniprot ID | PRTN3_HUMAN |
| Accession Number | NP_002768.3 |
| Immunogen Region | 88-98 |
| Immunogen Sequence | HNVRTQEPTQQ |
Additional Info
| Function | Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration. May play a role in neutrophil transendothelial migration, probably when associated with CD177. |
| Protein Name | MyeloblastinAgp7C-Anca AntigenLeukocyte Proteinase 3Pr-3Pr3Neutrophil Proteinase 4Np-4P29Wegener Autoantigen |
| Database Links | Reactome: R-HSA-140875Reactome: R-HSA-449836Reactome: R-HSA-6798695Reactome: R-HSA-6803157 |
| Cellular Localisation | Cytoplasmic GranuleSecretedCell MembranePeripheral Membrane ProteinExtracellular SideMembrane RaftLocalizes Predominantly To Azurophil Granules (Primary Secretory Granules) In NeutrophilsSecreted Upon Neutrophil Stimulation By Tnf-AlphaLipopolysaccharide (Lps)Fmlp And Cxcl8/Il8 Or During Neutrophil TransmigrationFollowing Secretion Tethered To The Cell Membrane By Cd177 |
| Alternative Antibody Names | Anti-Myeloblastin antibodyAnti-Agp7 antibodyAnti-C-Anca Antigen antibodyAnti-Leukocyte Proteinase 3 antibodyAnti-Pr-3 antibodyAnti-Pr3 antibodyAnti-Neutrophil Proteinase 4 antibodyAnti-Np-4 antibodyAnti-P29 antibodyAnti-Wegener Autoantigen antibodyAnti-PRTN3 antibodyAnti-MBN antibody |
Information sourced from Uniprot.org