![Western blot analysis of lysates from wild type (WT) and CD133 knockout (KO) HCT116 cells, using [KO Validated] CD133 Rabbit polyclonal antibody (STJ114584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93685/374848/STJ114584_2__18690.1713141458.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CD133 knockout (KO) HCT116 cells, using [KO Validated] CD133 Rabbit polyclonal antibody (STJ114584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western blot analysis of lysates from HCT116 cells, using [KO Validated] CD133 Rabbit polyclonal antibody (STJ114584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93685/374849/STJ114584_3__86070.1713141459.jpg?c=1 "Western blot analysis of lysates from HCT116 cells, using [KO Validated] CD133 Rabbit polyclonal antibody (STJ114584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
| Host: | Rabbit |
| Applications: | WB/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IF/ICC:1:50-1:200 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PROM1 |
| Gene ID: | 8842 |
| Uniprot ID: | PROM1_HUMAN |
| Immunogen Region: | 179-433 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 179-433 of human CD133 (NP_006008.1). |
| Immunogen Sequence: | ANHQVRTRIKRSRKLADSNF KDLRTLLNETPEQIKYILAQ YNTTKDKAFTDLNSINSVLG GGILDRLRPNIIPVLDEIKS MATAIKETKEALENMNSTLK SLHQQSTQLSSSLTSVKTSL RSSLNDPLCLVHPSSETCNS IRLSLSQLNSNPELRQLPPV DAELDNVNNVLRTDLDGLVQ QGYQSLNDIPDRVQRQTTTV VAGIKRVLNSIGSDIDNVTQ RLPIQDILSAFSVYVNNTE |
| Tissue Specificity | Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level). |
| Post Translational Modifications | Isoform 1 and isoform 2 are glycosylated. Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis. |
| Function | May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. |
| Protein Name | Prominin-1 Antigen Ac133 Prominin-Like Protein 1 Cd Antigen Cd133 |
| Cellular Localisation | Apical Cell Membrane Multi-Pass Membrane Protein Cell Projection Microvillus Membrane Cilium Photoreceptor Outer Segment Endoplasmic Reticulum Endoplasmic Reticulum-Golgi Intermediate Compartment Found In Extracellular Membrane Particles In Various Body Fluids Such As Cerebrospinal Fluid Saliva Seminal Fluid And Urine |
| Alternative Antibody Names | Anti-Prominin-1 antibody Anti-Antigen Ac133 antibody Anti-Prominin-Like Protein 1 antibody Anti-Cd Antigen Cd133 antibody Anti-PROM1 antibody Anti-PROML1 antibody Anti-MSTP061 antibody |
Information sourced from Uniprot.org