![Western blot analysis of lysates from wild type (WT) and CD133 knockout (KO) HCT116 cells, using [KO Validated] CD133 Rabbit polyclonal antibody (STJ114584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93685/374848/STJ114584_2__18690.1713141458.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CD133 knockout (KO) HCT116 cells, using [KO Validated] CD133 Rabbit polyclonal antibody (STJ114584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western blot analysis of lysates from HCT116 cells, using [KO Validated] CD133 Rabbit polyclonal antibody (STJ114584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93685/374849/STJ114584_3__86070.1713141459.jpg?c=1 "Western blot analysis of lysates from HCT116 cells, using [KO Validated] CD133 Rabbit polyclonal antibody (STJ114584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
Anti-PROM1 antibody (179-433) (STJ114584)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CD133 (179-433) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PROM1 |
| Gene ID | 8842 |
| Uniprot ID | PROM1_HUMAN |
| Immunogen Region | 179-433 |
| Immunogen Sequence | ANHQVRTRIKRSRKLADSNF KDLRTLLNETPEQIKYILAQ YNTTKDKAFTDLNSINSVLG GGILDRLRPNIIPVLDEIKS MATAIKETKEALENMNSTLK SLHQQSTQLSSSLTSVKTSL RSSLNDPLCLVHPSSETCNS IRLSLSQLNSNPELRQLPPV DAELDNVNNVLRTDLDGLVQ QGYQSLNDIPDRVQRQTTTV VAGIKRVLNSIGSDIDNVTQ RLPIQDILSAFSVYVNNTE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 179-433 of human CD133 (NP_006008.1). |
Additional Info
| Tissue Specificity | Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level). |
| Post Translational Modifications | Isoform 1 and isoform 2 are glycosylated. Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis. |
| Function | May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. |
| Protein Name | Prominin-1Antigen Ac133Prominin-Like Protein 1Cd Antigen Cd133 |
| Database Links | |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinCell ProjectionMicrovillus MembraneCiliumPhotoreceptor Outer SegmentEndoplasmic ReticulumEndoplasmic Reticulum-Golgi Intermediate CompartmentFound In Extracellular Membrane Particles In Various Body Fluids Such As Cerebrospinal FluidSalivaSeminal Fluid And Urine |
| Alternative Antibody Names | Anti-Prominin-1 antibodyAnti-Antigen Ac133 antibodyAnti-Prominin-Like Protein 1 antibodyAnti-Cd Antigen Cd133 antibodyAnti-PROM1 antibodyAnti-PROML1 antibodyAnti-MSTP061 antibody |
Information sourced from Uniprot.org