![Immunofluorescence analysis of HeLa cells using [KO Validated] PRMT2 Rabbit polyclonal antibody (STJ28398). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101688/389745/STJ28398_1__85710.1713159476.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KO Validated] PRMT2 Rabbit polyclonal antibody (STJ28398). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.")
![Immunohistochemistry analysis of PRMT2 in paraffin-embedded human liver damage using [KO Validated] PRMT2 Rabbit polyclonal antibody (STJ28398) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101688/389746/STJ28398_2__17117.1713159476.jpg?c=1 "Immunohistochemistry analysis of PRMT2 in paraffin-embedded human liver damage using [KO Validated] PRMT2 Rabbit polyclonal antibody (STJ28398) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and PRMT2 knockout (KO) 293T cells, using [KO Validated] PRMT2 Rabbit polyclonal antibody (STJ28398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101688/389747/STJ28398_3__22801.1713159477.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PRMT2 knockout (KO) 293T cells, using [KO Validated] PRMT2 Rabbit polyclonal antibody (STJ28398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western blot analysis of lysates from mouse lung, using [KO Validated] PRMT2 Rabbit polyclonal antibody (STJ28398) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101688/389748/STJ28398_4__63273.1713159478.jpg?c=1 "Western blot analysis of lysates from mouse lung, using [KO Validated] PRMT2 Rabbit polyclonal antibody (STJ28398) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.")
![Immunofluorescence analysis of HeLa cells using [KO Validated] PRMT2 Rabbit polyclonal antibody (STJ28398). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/101688/389745/STJ28398_1__85710.1713159476.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KO Validated] PRMT2 Rabbit polyclonal antibody (STJ28398). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.")
Anti-PRMT2 antibody (1-300) (STJ28398)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PRMT2 (1-300) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PRMT2 |
| Gene ID | 3275 |
| Uniprot ID | ANM2_HUMAN |
| Immunogen Region | 1-300 |
| Immunogen Sequence | MATSGDCPRSESQGEEPAEC SEAGLLQEGVQPEEFVAIAD YAATDETQLSFLRGEKILIL RQTTADWWWGERAGCCGYIP ANHVGKHVDEYDPEDTWQDE EYFGSYGTLKLHLEMLADQP RTTKYHSVILQNKESLTDKV ILDVGCGTGIISLFCAHYAR PRAVYAVEASEMAQHTGQLV LQNGFADIITVYQQKVEDVV LPEKVDVLVSEWMGTCLLFE FMIESILYARDAWLKEDGV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PRMT2 (NP_996845.1). |
Additional Info
| Tissue Specificity | Widely expressed. Highly expressed in androgen target organs such as heart, prostate, skeletal muscle, ovary and spinal cord. |
| Function | Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation. |
| Protein Name | Protein Arginine N-Methyltransferase 2Histone-Arginine N-Methyltransferase Prmt2 |
| Database Links | |
| Cellular Localisation | Isoform 1: CytoplasmNucleusTranslocates From The Cytoplasm To The NucleusAfter Hormone ExposureExcluded From NucleolusIsoform Prmt2alpha: NucleusIsoform Prmt2beta: CytoplasmNucleolusIsoform Prmt2gamma: NucleusIsoform Prmt2l2: CytoplasmPredominantly Cytoplasmic |
| Alternative Antibody Names | Anti-Protein Arginine N-Methyltransferase 2 antibodyAnti-Histone-Arginine N-Methyltransferase Prmt2 antibodyAnti-PRMT2 antibodyAnti-HMT1 antibodyAnti-HRMT1L1 antibody |
Information sourced from Uniprot.org