Anti-PRKG1 antibody (609-659) {Biotin} (STJ502529)

SKU:
STJ502529-100

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Host: Rabbit
Applications: ELISA/IP/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRKG1 (609-659) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Purification: Affinity Purified
Concentration: 0.64-0.72 µg/µl
Dilution Range: WB: 1:500
DB: 1:10, 000
ELISA: 1:10, 000
IP: 1:200
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: PRKG1
Gene ID: 5592
Uniprot ID: KGP1_HUMAN
Immunogen Region: 609-659
Immunogen: Synthetic peptide taken within amino acid region 609-659 on human protein kinase G alpha subunit.
Tissue Specificity Primarily expressed in lung and placenta.
Post Translational Modifications Autophosphorylation increases kinase activity. 65 kDa monomer is produced by proteolytic cleavage.
Function Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types. Proteins that are phosphorylated by PRKG1 regulate platelet activation and adhesion, smooth muscle contraction, cardiac function, gene expression, feedback of the NO-signaling pathway, and other processes involved in several aspects of the CNS like axon guidance, hippocampal and cerebellar learning, circadian rhythm and nociception. Smooth muscle relaxation is mediated through lowering of intracellular free calcium, by desensitization of contractile proteins to calcium, and by decrease in the contractile state of smooth muscle or in platelet activation. Regulates intracellular calcium levels via several pathways: phosphorylates IRAG1 and inhibits IP3-induced Ca(2+) release from intracellular stores, phosphorylation of KCNMA1 (BKCa) channels decreases intracellular Ca(2+) levels, which leads to increased opening of this channel. PRKG1 phosphorylates the canonical transient receptor potential channel (TRPC) family which inactivates the associated inward calcium current. Another mode of action of NO/cGMP/PKGI signaling involves PKGI-mediated inactivation of the Ras homolog gene family member A (RhoA). Phosphorylation of RHOA by PRKG1 blocks the action of this protein in myriad processes: regulation of RHOA translocation.decreasing contraction.controlling vesicle trafficking, reduction of myosin light chain phosphorylation resulting in vasorelaxation. Activation of PRKG1 by NO signaling alters also gene expression in a number of tissues. In smooth muscle cells, increased cGMP and PRKG1 activity influence expression of smooth muscle-specific contractile proteins, levels of proteins in the NO/cGMP signaling pathway, down-regulation of the matrix proteins osteopontin and thrombospondin-1 to limit smooth muscle cell migration and phenotype. Regulates vasodilator-stimulated phosphoprotein (VASP) functions in platelets and smooth muscle.
Protein Name Cgmp-Dependent Protein Kinase 1
Cgk 1
Cgk1
Cgmp-Dependent Protein Kinase I
Cgki
Database Links Reactome: R-HSA-392517 Q13976-1
Reactome: R-HSA-4086398
Reactome: R-HSA-418457 Q13976-1
Cellular Localisation Cytoplasm
Colocalized With Trpc7 In The Plasma Membrane
Alternative Antibody Names Anti-Cgmp-Dependent Protein Kinase 1 antibody
Anti-Cgk 1 antibody
Anti-Cgk1 antibody
Anti-Cgmp-Dependent Protein Kinase I antibody
Anti-Cgki antibody
Anti-PRKG1 antibody
Anti-PRKG1B antibody
Anti-PRKGR1A antibody
Anti-PRKGR1B antibody

Information sourced from Uniprot.org

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